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Journal article

Secretory products from human adipocytes impair endothelial function via nuclear factor kappa B

Authors list: Kralisch, S; Sommer, G; Stangl, V; Köhler, U; Kratzch, J; Stepan, H; Faber, R; Schubert, A; Lössner, U; Vietzke, A; Blüher, M; Stumvoll, M; Fasshauer, M

Publication year: 2008

Pages: 523-531

Journal: Atherosclerosis

Volume number: 196

Issue number: 2

ISSN: 0021-9150

DOI Link: https://doi.org/10.1016/j.atherosclerosis.2007.05.016

Publisher: Elsevier

Abstract:
Hyperplasia and hypertrophy of fat cells can be found in obesity, and increased adiposity is associated with endothelial dysfunction as an early event of atherosclerosis. However, it is unclear whether human adipocytes directly influence endothelial function. To study the crosstalk between fat and endothelial cells, human umbilical venous endothelial cells (HUVECs), and human coronary artery endothelial cells (HCAECs) were cultured in infranatants (Adipo) of primary differentiated human adipocytes. Interestingly, incubation of HUVECs and HCAECs with Adipo significantly increased monocyte adhesion 7.3 and 2.2-fold, respectively. VCAM-1, ICAM-1, and E-selectin in HUVECs were upregulated 3.9, 3.0, and 9.5-fold, respectively, under these conditions. Furthermore, Adipo significantly stimulated NF kappa B activity 1.9-fold. The NF kappa B inhibitor MG-132 and heat inactivation significantly reversed Adipo-stimulated monocyte adhesion. TNF alpha-neutralizing antibodies partly reversed Adipo-induced monocyte adhesion. In contrast, thiazolidinedione-pretreatment of human adipocytes did not alter the effects of Adipo. Adipo did not show cytotoxic effects. Taken together, we demonstrate that endothelial dysfunction is induced by adipocyte-secreted factors via NF kappa B partly dependent on TNF alpha. (c) 2007 Elsevier Ireland Ltd. All rights reserved.

Authors/Editors

- Uni.-Prof. Dr. Mathias Faßhauer

Citation Styles

Harvard Citation style: Kralisch, S., Sommer, G., Stangl, V., Köhler, U., Kratzch, J., Stepan, H., et al. (2008) Secretory products from human adipocytes impair endothelial function via nuclear factor kappa B, Atherosclerosis, 196(2), pp. 523-531. https://doi.org/10.1016/j.atherosclerosis.2007.05.016

APA Citation style: Kralisch, S., Sommer, G., Stangl, V., Köhler, U., Kratzch, J., Stepan, H., Faber, R., Schubert, A., Lössner, U., Vietzke, A., Blüher, M., Stumvoll, M., & Fasshauer, M. (2008). Secretory products from human adipocytes impair endothelial function via nuclear factor kappa B. Atherosclerosis. 196(2), 523-531. https://doi.org/10.1016/j.atherosclerosis.2007.05.016