Journal article

Tissue inhibitor of metalloproteinase-1 mRNA production and protein secretion are induced by interleukin-1 beta in 3T3-L1 adipocytes


Authors listWeise, S; Kralisch, S; Sommer, G; Lössner, U; Blüher, M; Stumvoll, M; Fasshauer, M

Publication year2008

Pages169-174

JournalJournal of Endocrinology

Volume number198

Issue number1

ISSN0022-0795

DOI Linkhttps://doi.org/10.1677/JOE-07-0631

PublisherBioScientifica


Abstract
The adipokine tissue inhibitor of metalloproteinase (TIMP)-1 is upregulated when weight is gained and promotes adipose tissue development. In the present study, the effect of insulin resistance-inducing and proinflammatory interleukin (IL)-1 beta on TIMP-1 gene expression and secretion was investigated in 3T3-L1 adipocytes. Interestingly, protein secretion and mRNA production of TIMP-1 were significantly stimulated by IL-1 beta. Thus, IL-1 beta induced TIMP-1 secretion in a dose-dependent manner with maximal 3.5-fold upregulation seen at 0.67 ng/ml IL-1 beta relative to untreated cells. Furthermore, TIMP-1 mRNA synthesis was significantly stimulated by IL-1 beta in a dose-dependent fashion with 2.5-fold induction seen at IL-1 beta concentrations as low as 0.02 ng/ml and maximal 8.1-fold upregulation found at 20 ng/ml effector. Induction of TIMP-1 mRNA was also time dependent with maximal 9.6-fold upregulation detectable after 8 h of IL-1 beta treatment. Signaling studies suggested that janus kinase 2 is involved in IL-1 beta-induced TIMP-1 mRNA expression. Taken together, our results demonstrate that the TIMP-1 expression is selectively upregulated by proinflammatory IL-1 beta, supporting a direct association between insulin resistance, inflammation, and adipose tissue development in obesity.



Citation Styles

Harvard Citation styleWeise, S., Kralisch, S., Sommer, G., Lössner, U., Blüher, M., Stumvoll, M., et al. (2008) Tissue inhibitor of metalloproteinase-1 mRNA production and protein secretion are induced by interleukin-1 beta in 3T3-L1 adipocytes, Journal of Endocrinology, 198(1), pp. 169-174. https://doi.org/10.1677/JOE-07-0631

APA Citation styleWeise, S., Kralisch, S., Sommer, G., Lössner, U., Blüher, M., Stumvoll, M., & Fasshauer, M. (2008). Tissue inhibitor of metalloproteinase-1 mRNA production and protein secretion are induced by interleukin-1 beta in 3T3-L1 adipocytes. Journal of Endocrinology. 198(1), 169-174. https://doi.org/10.1677/JOE-07-0631


Last updated on 2025-21-05 at 16:22