Journal article

Publication year: 2009

Pages: 102-106

Journal: Regulatory Peptides

Volume number: 154

Issue number: 1-3

ISSN: 0167-0115

DOI Link: https://doi.org/10.1016/j.regpep.2009.02.010

Publisher: Elsevier

Abstract: 
Chemerin has recently been characterized as a novel adipokine playing a crucial role in adipocyte differentiation and insulin signalling. In the current study, the impact of insulin resistance-inducing and proinflammatory interleukin (IL)-1 beta on chemerin protein secretion and mRNA expression was determined in 3T3-L1 adipocytes. Interestingly, IL-1 beta significantly induced chemerin protein secretion almost 1.3-fold from 5.89 ng/ml (basal) to 7.52 ng/ml. Furthermore, chemerin mRNA synthesis was significantly stimulated by IL-1 beta in a dose-dependent fashion with 1.5-fold induction seen at IL-1 beta concentrations as low as 0.07 ng/ml and maximal 2.6-fold upregulation found at 2 ng/ml effector. Induction of chemerin mRNA by IL-1 beta was time-dependent in both 3T3-L1 adipocytes and brown fat cells. Signalling studies suggested that Janus kinase 2, nuclear factor kappa B, p44/42 mitogen-activated protein kinase, and phosphatidylinositol 3-kinase are involved in IL-1 beta-induced chemerin mRNA expression. Furthermore, recombinant chemerin downregulated insulin-stimulated glucose uptake. Taken together, we show that chemerin is upregulated in fat cells by IL-1 beta and might modulate the effects of IL-1 beta on adipocyte metabolism and insulin sensitivity. (C) 2009 Elsevier B.V. All rights reserved.

Harvard Citation style: Kralisch, S., Weise, S., Sommer, G., Lipfert, J., Lössner, U., Blüher, M., et al. (2009) Interleukin-1 beta induces the novel adipokine chemerin in adipocytes in vitro, Regulatory Peptides, 154(1-3), pp. 102-106. https://doi.org/10.1016/j.regpep.2009.02.010

APA Citation style: Kralisch, S., Weise, S., Sommer, G., Lipfert, J., Lössner, U., Blüher, M., Stumvoll, M., & Fasshauer, M. (2009). Interleukin-1 beta induces the novel adipokine chemerin in adipocytes in vitro. Regulatory Peptides. 154(1-3), 102-106. https://doi.org/10.1016/j.regpep.2009.02.010