Secretory Products From Human Adipocytes Stimulate Proinflammatory Cytokine Secretion From Human Endothelial Cells - Research portal of Justus Liebig University Giessen:

Journal article

Secretory Products From Human Adipocytes Stimulate Proinflammatory Cytokine Secretion From Human Endothelial Cells

Authors list: Sommer, G; Kralisch, S; Stangl, V; Vietzke, A; Köhler, U; Stepan, H; Faber, R; Schubert, A; Lössner, U; Blüher, M; Stumvoll, M; Fasshauer, M

Publication year: 2009

Pages: 729-737

Journal: Journal of Cellular Biochemistry

Volume number: 106

Issue number: 4

ISSN: 0730-2312

DOI Link: https://doi.org/10.1002/jcb.22068

Publisher: Wiley

Abstract:
Hyperplasia and hypertrophy of rat cells can be found in obesity and increased adiposity is associated with endothelial dysfunction as an early event of atherosclerosis. However, it is unclear whether human adipocytes directly influence endothelial protein secretion. To study the crosstalk between rat and endothelial cells, human umbilical venous endothelial cells (HUVECs) were cultured in infranatants (Adipo) of primary differentiated human adipocytes. Interestingly, significantly increased secretion of 23 cytokines and chemokines from HUVECs was detected in lour independent experiments after Adipo stimulation by protein array analysis detecting a total of 174 different proteins. Among those, time-dependent Adipo-induced upregulation of cytokine secretion in HUVECs was confirmed by ELISA for interleukin (IL)-8, monokine induced by gamma interferon, macrophage inflammatory protein (MIP)-1 beta, MIP-3 alpha, monocyte chemoattractant protein-1, and IL-6. Factors besides adiponectin, leptin, resistin, and tumor necrosis factor alpha appear to mediate these stimulatory effects. Our findings suggest that endothelial cell secretion is significantly influenced towards a proinflammatory pattern by adipocyte-secreted factors. J. Cell. Biochem. 106: 729-737, 2009. (C) 2009 Wiley-Liss, Inc.

Authors/Editors

- Uni.-Prof. Dr. Mathias Faßhauer

Citation Styles

Harvard Citation style: Sommer, G., Kralisch, S., Stangl, V., Vietzke, A., Köhler, U., Stepan, H., et al. (2009) Secretory Products From Human Adipocytes Stimulate Proinflammatory Cytokine Secretion From Human Endothelial Cells, Journal of Cellular Biochemistry, 106(4), pp. 729-737. https://doi.org/10.1002/jcb.22068

APA Citation style: Sommer, G., Kralisch, S., Stangl, V., Vietzke, A., Köhler, U., Stepan, H., Faber, R., Schubert, A., Lössner, U., Blüher, M., Stumvoll, M., & Fasshauer, M. (2009). Secretory Products From Human Adipocytes Stimulate Proinflammatory Cytokine Secretion From Human Endothelial Cells. Journal of Cellular Biochemistry. 106(4), 729-737. https://doi.org/10.1002/jcb.22068