Journal article

Publication year: 2006

Pages: 56-62

Journal: Molecular and Cellular Endocrinology

Volume number: 253

Issue number: 1-2

ISSN: 0303-7207

DOI Link: https://doi.org/10.1016/j.mce.2006.04.027

Publisher: Elsevier

Abstract: 
Various adipocytokines have been described which influence insulin sensitivity and vascular function profoundly and might, therefore, potentially link obesity, insulin resistance, and atherosclerosis. Among those, plasminogen activator inhibitor (PAI)-1 is an adipose-secreted factor upregulated in obesity and insulin resistance that inhibits fibrinolysis. Furthermore, recent studies in knockout mice suggest that PAI-1 directly impairs insulin sensitivity. In the current study, the impact of growth hormone (GH) and interleukin (IL)-6 on PAI-1 mRNA synthesis and secretion was determined in 3T3-L1 adipocytes. Interestingly, 500 ng/ml GH and 30 ng/ml IL-6 increased PAI-1 secretion five-fold and 3.6-fold, respectively. Furthermore, GH and IL-6 induced PAI-1 mRNA by up to 7.3-fold, and 3.6-fold, respectively, in a time-dependent fashion with significant stimulation seen at concentrations as low as 5 ng/ml GH and 10 ng/ml IL-6. Other insulin resistance-inducing hormones which stimulated PAI-1 synthesis included insulin, TNF alpha, and dexamethasone. Studies using pharmacological inhibitors suggested that basal and GH-induced PAI-1 synthesis were at least in part mediated by p44/42 mitogen-activated protein kinase but not janus kinase 2 and phosphatidylinositol 3-kinase. Taken together, our results show a differential regulation of PAI-1 mRNA by insulin resistance-inducing hormones including GH and IL-6. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

Harvard Citation style: Kralisch, S., Klein, J., Lössner, U., Blüher, M., Paschke, R., Stumvoll, M., et al. (2006) Plasminogen activator inhibitor-1 expression and secretion are stimulated by growth hormone and interleukin-6 in 3T3-L1 adipocytes, Molecular and Cellular Endocrinology, 253(1-2), pp. 56-62. https://doi.org/10.1016/j.mce.2006.04.027

APA Citation style: Kralisch, S., Klein, J., Lössner, U., Blüher, M., Paschke, R., Stumvoll, M., & Fasshauer, M. (2006). Plasminogen activator inhibitor-1 expression and secretion are stimulated by growth hormone and interleukin-6 in 3T3-L1 adipocytes. Molecular and Cellular Endocrinology. 253(1-2), 56-62. https://doi.org/10.1016/j.mce.2006.04.027