Journalartikel

Jahr der Veröffentlichung: 2006

Seiten: 665-670

Zeitschrift: Journal of Endocrinology

Bandnummer: 189

Heftnummer: 3

ISSN: 0022-0795

DOI Link: https://doi.org/10.1677/joe.1.06645

Verlag: BioScientifica

Abstract: 
Tissue inhibitor of metalloproteinase (TIMP)-1 is an adipocytokine upregulated in obesity which might promote adipose tissue development. In the current study, the impact of the beta-adrenergic agonist isoproterenol on TIMP-1 gene expression and secretion was determined in 3T3-L1 adipocytes. Interestingly, isoproterenol increased TIMP-1 secretion 2.7-fold. Furthermore, isoproterenol induced TIMP-1 mRNA in a time- and dose-dependent fashion with significant effects observed as early as 1 h after effector addition and at concentrations as low as 1 mu M isoproterenol. Significant isoproterenol-induced upregulation of TIMP-1 mRNA could also be found in immortalized brown adipocytes. Inhibitor experiments confirmed that the positive effect of isoproterenol on TIMP-1 is mediated via P-adrenergic receptors and protein kinase A. Moreover, increasing cAMP levels with forskolin or dibutyryl-cAMP was sufficient to stimulate TIMP-1 synthesis. Insulin induced basal TIMP-1 mRNA, but did not significantly influence forskohn-induced TIMP-1 expression. Taken together, we demonstrate that TIMP-1 expression and secretion are selectively upregulated in adipocytes by beta-adrenergic agonists via a classic Gs-protein-coupled pathway.

Harvard-Zitierstil: Kralisch, S., Lössner, U., Blüher, M., Paschke, R., Stumvoll, M. and Fasshauer, M. (2006) Tissue inhibitor of metalloproteinase 1 expression and secretion are induced by beta-adrenergic stimulation in 3T3-L1 adipocytes, Journal of Endocrinology, 189(3), pp. 665-670. https://doi.org/10.1677/joe.1.06645

APA-Zitierstil: Kralisch, S., Lössner, U., Blüher, M., Paschke, R., Stumvoll, M., & Fasshauer, M. (2006). Tissue inhibitor of metalloproteinase 1 expression and secretion are induced by beta-adrenergic stimulation in 3T3-L1 adipocytes. Journal of Endocrinology. 189(3), 665-670. https://doi.org/10.1677/joe.1.06645