Journal article

Publication year: 2003

Pages: 1045-1050

Journal: Biochemical and Biophysical Research Communications

Volume number: 301

Issue number: 4

ISSN: 0006-291X

DOI Link: https://doi.org/10.1016/S0006-291X(03)00090-1

Publisher: Elsevier

Abstract: 
Recently, it has been shown that adiponectin is an important insulin-sensitizing fat-derived protein which is downregulated in insulin resistance and obesity, and replenishment of which improves insulin sensitivity. In contrast, interleukin (IL)-6 appears as an adipocytokine serum concentrations of which are elevated in these states. However, it has not been determined whether IL-6 might impact on expression and secretion of adiponectin. To clarify this, 3T3-L1 adipocytes were treated with different concentrations of IL-6 for various periods of time. Adiponectin mRNA was measured by quantitative real-time reverse transcription-polymerase chain reaction and secretion was determined by radioimmunoassays. Interestingly, treatment of 3T3-L1 cells with 30ng/ml IL-6 significantly decreased adiponectin secretion to 75% of control levels. Adiponectin secretion was also inhibited between 25% and 45% by chronic treatment with forskolin (50muM), tumor necrosis factor a (100ng/ml), and dexamethasone (100nM). Furthermore, adiponectin mRNA expression was downregulated by up to 50% in a time- and dose-dependent manner, with significant inhibition detectable at concentrations as low as 3ng/ml IL-6 and as early as 8 h after effector addition. The inhibitory effect of IL-6 was partially reversed by pretreatment of 3T3-L1 cells with pharmacological inhibitors of a p44/42 mitogen-activated protein (MAP) kinase. Moreover, the negative effect of IL-6 on adiponectin mRNA expression could be reversed by withdrawal of the hormone for 24h. Taken together, our results suggest that adiponectin gene expression is reversibly downregulated by IL-6 and support the concept of adiponectin being an important selectively controlled modulator of insulin sensitivity. (C) 2003 Elsevier Science (USA). All rights reserved.

Harvard Citation style: Fasshauer, M., Kralisch, S., Klier, M., Lössner, U., Blüher, M., Klein, J., et al. (2003) Adiponectin gene expression and secretion is inhibited by interleukin-6 in 3T3-L1 adipocytes, Biochemical and Biophysical Research Communications, 301(4), pp. 1045-1050. https://doi.org/10.1016/S0006-291X(03)00090-1

APA Citation style: Fasshauer, M., Kralisch, S., Klier, M., Lössner, U., Blüher, M., Klein, J., & Paschke, R. (2003). Adiponectin gene expression and secretion is inhibited by interleukin-6 in 3T3-L1 adipocytes. Biochemical and Biophysical Research Communications. 301(4), 1045-1050. https://doi.org/10.1016/S0006-291X(03)00090-1