Journal article

Publication year: 2012

Pages: 1450-1455

Journal: African Journal of Agricultural Research

Volume number: 7

Issue number: 10

DOI Link: https://doi.org/10.5897/AJAR11.1014

Publisher: Academic Journals

Abstract: 

In this study, the hypothesis was investigated that activation of peroxisome proliferator-activated receptor (PPAR)-α regulates homeostasis of carnitine in laying hens. Therefore, laying hens received  either a control diet or a diet supplemented with 0.15% clofibrate as a synthetic PPARα agonist for 4  weeks. Feed intake was not different between both groups of hens while egg production rate was slightly reduced in the group of hens treated with clofibrate (P < 0.05). Hens treated with clofibrate had an increased expression of the classical PPARα target genes carnitine palmitoyltransferase-1 and acyl CoA oxidase in the liver compared to control hens (P < 0.05), indicative of an activation of PPARα. In hens treated with clofibrate, mRNA concentration of novel organic cation transporter (OCTN)-2, the most important carnitine transporter, in the liver as well as carnitine concentrations in plasma, liver, egg yolk and albumen were increased compared to control hens (P < 0.05). mRNA concentrations of enzymes of hepatic carnitine synthesis as well as concentrations of the carnitine precursors trimethyllysine and γ-butyrobetaine in plasma, liver and muscle were unchanged in hens treated with clofibrate, suggesting that activation of PPARα did not influence carnitine biosynthesis. In conclusion, this study shows that activation of PPARα up-regulates expression of OCTN2 in the liver of laying hens, such as in mammalian species and causes an increase of carnitine concentrations in liver, plasma and egg.

Harvard Citation style: Shibani, M., Keller, J., Kluge, H., König, B., Stangl, G., Ringseis, R., et al. (2012) Effects of activation of peroxisome proliferator-activated receptor-α by clofibrate on carnitine homeostasis in laying hens, African Journal of Agricultural Research, 7(10), pp. 1450-1455. https://doi.org/10.5897/AJAR11.1014

APA Citation style: Shibani, M., Keller, J., Kluge, H., König, B., Stangl, G., Ringseis, R., & Eder, K. (2012). Effects of activation of peroxisome proliferator-activated receptor-α by clofibrate on carnitine homeostasis in laying hens. African Journal of Agricultural Research. 7(10), 1450-1455. https://doi.org/10.5897/AJAR11.1014