Journal article

Direct and individual analysis of stress-related phytohormone dispersion in the vascular system of Cucurbita maxima after flagellin 22 treatment


Authors list: Furch, ACU; Zimmermann, MR; Kogel, KH; Reichelt, M; Mithofer, A

Publication year: 2014

Pages: 1176-1182

Journal: New Phytologist

Volume number: 201

Issue number: 4

ISSN: 0028-646X

Open access status: Green

DOI Link: https://doi.org/10.1111/nph.12661

Publisher: Wiley


Abstract: 
The stress-related phytohormones, salicylic acid (SA) and abscisic acid (ABA), and the three jasmonates, jasmonic acid (JA), cis-12-oxo-phytodienoic acid (cis-OPDA), and (+)-7-iso-jasmonoyl-L-isoleucine (JA-Ile), were investigated in phloem and xylem exudates of Cucurbita maxima.Phloem and xylem exudates were separately collected and analysed via liquid chromatography-mass spectrometry.We show direct evidence for all three jasmonates, ABA, and SA in both phloem and xylem exudates of C. maxima. JA and JA-Ile concentrations are higher in xylem (JA: c(xylem) approximate to 199.5 nM, c(phloem) approximate to 43.9 nM; JA-Ile: c(xylem) approximate to 7.9 nM, c(phloem) approximate to 1.6 nM), whereas ABA and SA concentrations are higher in phloem exudates (ABA: c(xylem) approximate to 37.1 nM, c(phloem) approximate to 142.6 nM; SA: c(xylem) approximate to 61.6 nM, c(phloem) approximate to 1319 nM). During bacteria-derived flagellin 22 (flg22)-triggered remote root-to-shoot signalling, phytohormone concentration changed rapidly both in phloem and xylem.The unequal distribution of phytohormones suggests that phloem and xylem have distinct roles in defence responses. Our data shed light on systemic phytohormone signalling and help explain how plants cope with environmental challenges by lateral exchange between phloem and xylem. Our analysis is a starting point for further investigations of how phytohormones contribute to phloem- and xylem-based defence signalling.



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Citation Styles

Harvard Citation style: Furch, A., Zimmermann, M., Kogel, K., Reichelt, M. and Mithofer, A. (2014) Direct and individual analysis of stress-related phytohormone dispersion in the vascular system of Cucurbita maxima after flagellin 22 treatment, New Phytologist, 201(4), pp. 1176-1182. https://doi.org/10.1111/nph.12661

APA Citation style: Furch, A., Zimmermann, M., Kogel, K., Reichelt, M., & Mithofer, A. (2014). Direct and individual analysis of stress-related phytohormone dispersion in the vascular system of Cucurbita maxima after flagellin 22 treatment. New Phytologist. 201(4), 1176-1182. https://doi.org/10.1111/nph.12661


Last updated on 2025-10-06 at 10:21