The Mutualistic Fungus Piriformospora indica Colonizes Arabidopsis Roots by Inducing an Endoplasmic Reticulum Stress-Triggered Caspase-Dependent Cell Death - Research portal of Justus Liebig University Giessen:

Journal article

The Mutualistic Fungus Piriformospora indica Colonizes Arabidopsis Roots by Inducing an Endoplasmic Reticulum Stress-Triggered Caspase-Dependent Cell Death

Authors list: Qiang, XY; Zechmann, B; Reitz, MU; Kogel, KH; Schäfer, P

Publication year: 2012

Pages: 794-809

Journal: Plant Cell

Volume number: 24

Issue number: 2

ISSN: 1040-4651

Open access status: Green

DOI Link: https://doi.org/10.1105/tpc.111.093260

Publisher: Oxford University Press

Abstract:
In Arabidopsis thaliana roots, the mutualistic fungus Piriformospora indica initially colonizes living cells, which die as the colonization proceeds. We aimed to clarify the molecular basis of this colonization-associated cell death. Our cytological analyses revealed endoplasmic reticulum (ER) swelling and vacuolar collapse in invaded cells, indicative of ER stress and cell death during root colonization. Consistent with this, P. indica-colonized plants were hypersensitive to the ER stress inducer tunicamycin. By clear contrast, ER stress sensors bZIP60 and bZIP28 as well as canonical markers for the ER stress response pathway, termed the unfolded protein response (UPR), were suppressed at the same time. Arabidopsis mutants compromised in caspase 1-like activity, mediated by cell death-regulating vacuolar processing enzymes (VPEs), showed reduced colonization and decreased cell death incidence. We propose a previously unreported microbial invasion strategy during which P. indica induces ER stress but inhibits the adaptive UPR. This disturbance results in a VPE/caspase 1-like-mediated cell death, which is required for the establishment of the symbiosis. Our results suggest the presence of an at least partially conserved ER stress-induced caspase-dependent cell death pathway in plants as has been reported for metazoans.

Authors/Editors

- Prof. Dr. Karl-Heinz Kogel

Citation Styles

Harvard Citation style: Qiang, X., Zechmann, B., Reitz, M., Kogel, K. and Schäfer, P. (2012) The Mutualistic Fungus Piriformospora indica Colonizes Arabidopsis Roots by Inducing an Endoplasmic Reticulum Stress-Triggered Caspase-Dependent Cell Death, Plant Cell, 24(2), pp. 794-809. https://doi.org/10.1105/tpc.111.093260

APA Citation style: Qiang, X., Zechmann, B., Reitz, M., Kogel, K., & Schäfer, P. (2012). The Mutualistic Fungus Piriformospora indica Colonizes Arabidopsis Roots by Inducing an Endoplasmic Reticulum Stress-Triggered Caspase-Dependent Cell Death. Plant Cell. 24(2), 794-809. https://doi.org/10.1105/tpc.111.093260

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