Journal article

Publication year: 2006

Pages: 543-552

Journal: Molecular Plant Pathology

Volume number: 7

Issue number: 6

ISSN: 1464-6722

Open access status: Hybrid

DOI Link: https://doi.org/10.1111/j.1364-3703.2006.00359.x

Publisher: Wiley

Abstract: 
BAX Inhibitor-1 (BI-1) is a conserved cell death suppressor protein. In barley, BI-1 (HvBI-1) expression is induced upon powdery mildew infection and when over-expressed in epidermal cells of barley, HvBI-1 induces susceptibility to the biotrophic fungal pathogen Blumeria graminis. We co-expressed mammalian pro-apoptotic BAX together with HvBI-1, and the mammalian BAX antagonist BCL-X-L in barley epidermal cells. BAX expression led to cessation of cytoplasmic streaming and collapse of the cytoplasm while co-expression of HvBI-1 and BCL-X-L partially or completely, respectively, rescued cells from BAX lethality. When B. graminis was attacking epidermal cells, a green fluorescent protein fusion of HvBI-1 accumulated at the site of attempted penetration and was also present around haustoria. Over-expression of HvBI-1 in epidermal cells weakened a cell-wall-associated local hydrogen peroxide burst in a resistant mlo-mutant genotype and supported haustoria accommodation in race-specifically resistant MLA12-barley. HvBI-1 is a cell death regulator protein of barley with the potential to suppress host defence reactions.

Harvard Citation style: Eichmann, R., Dechert, C., Kogel, K. and Hückelhoven, R. (2006) Transient over-expression of barley BAX Inhibitor-1 weakens oxidative defence and MLA12-mediated resistance to Blumeria graminis f.sp hordei, Molecular Plant Pathology, 7(6), pp. 543-552. https://doi.org/10.1111/j.1364-3703.2006.00359.x

APA Citation style: Eichmann, R., Dechert, C., Kogel, K., & Hückelhoven, R. (2006). Transient over-expression of barley BAX Inhibitor-1 weakens oxidative defence and MLA12-mediated resistance to Blumeria graminis f.sp hordei. Molecular Plant Pathology. 7(6), 543-552. https://doi.org/10.1111/j.1364-3703.2006.00359.x