Journal article

Publication year: 2006

Pages: 3781-3791

Journal: Journal of Experimental Botany

Volume number: 57

Issue number: 14

ISSN: 0022-0957

Open access status: Green

DOI Link: https://doi.org/10.1093/jxb/erl191

Publisher: Oxford University Press

Abstract: 
Reactive oxygen intermediates (ROI) are closely related to defence reactions of plants against pathogens. A prominent role in the production of ROI has been attributed to the plant respiratory burst oxidase homologues (RBOH) of the human phagocyte GP91(phox). A barley RBOH, which encodes a putative superoxide (O-2(.-)) producing NADPH oxidase, is described here. Histochemical analysis of the barley-Blumeria graminis f. sp. hordei (Bgh) interaction showed that O-2(.-) is produced locally at the site of penetration. In contrast, hydrogen peroxide (H2O2) is produced in non-penetrated cell wall appositions. A barley RBOHA cDNA was isolated and a minor induction of expression of RBOHA was observed during the interactions of barley with Bgh. Transient RNA interference-mediated gene silencing of HvRBOHA during the penetration process of Bgh led to an increase of basal penetration resistance. The results support a potential role of HvRBOHA in cellular accessibility to Blumeria graminis.

Harvard Citation style: Trujillo, M., Altschmied, L., Schweizer, P., Kogel, K. and Hückelhoven, R. (2006) Respiratory Burst Oxidase Homologue A of barley contributes to penetration by the powdery mildew fungus Blumeria graminis f. sp hordei, Journal of Experimental Botany, 57(14), pp. 3781-3791. https://doi.org/10.1093/jxb/erl191

APA Citation style: Trujillo, M., Altschmied, L., Schweizer, P., Kogel, K., & Hückelhoven, R. (2006). Respiratory Burst Oxidase Homologue A of barley contributes to penetration by the powdery mildew fungus Blumeria graminis f. sp hordei. Journal of Experimental Botany. 57(14), 3781-3791. https://doi.org/10.1093/jxb/erl191