Journalartikel

Jahr der Veröffentlichung: 2003

Seiten: 1275-1280

Zeitschrift: Plant Science: An international journal of experimental plant biology

Bandnummer: 165

Heftnummer: 6

ISSN: 0168-9452

DOI Link: https://doi.org/10.1016/S0168-9452(03)00336-4

Verlag: Elsevier

Abstract: 
The pathogenesis-related protein 1 (PR-1b) of barley (Hordeum vulgare L.) is a marker for the attack by the powdery mildew fungus (Blumeria graminis f.sp. hordei, Bgh) and other pathogens. PR-1b consists of 164 amino acids and has a potential signal peptide for export into the cell wall. Here, we show that PR-lb is differentially expressed in near-isogenic barley lines exhibiting various forms of defence phenotypes including papilla formation and the hypersensitive cell death. To elucidate PR-1b function, we transiently silenced PR-1 expression by double stranded RNA (dsRNA) interference in the moderately susceptible barley double mutant line A89 (genotype: mlo5-ror1), which shows a papillae-based defence phenotype. Upon bombardment of leaf segments with PR-1b dsRNA and a GFP marker gene construct, Bgh slightly more frequently penetrated the plant cell wall of transformed epidermal cells relative to cells bombarded with human control dsRNA. We conclude that PR-1b contributes to penetration resistance to the powdery mildew fungus in barley. We also observed that PR-1b expression correlates with the production of H2O2 in responses to Bgh and Bipolaris sorokiniana and was induced upon infiltration of the H2O2 producing mixture of glucose and glucose oxidase. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

Harvard-Zitierstil: Schultheiss, H., Dechert, C., Kiraly, L., Fodor, J., Michel, K., Kogel, K., et al. (2003) Functional assessment of the pathogenesis-related protein PR-1b in barley, Plant Science: An international journal of experimental plant biology, 165(6), pp. 1275-1280. https://doi.org/10.1016/S0168-9452(03)00336-4

APA-Zitierstil: Schultheiss, H., Dechert, C., Kiraly, L., Fodor, J., Michel, K., Kogel, K., & Hückelhoven, R. (2003). Functional assessment of the pathogenesis-related protein PR-1b in barley. Plant Science: An international journal of experimental plant biology. 165(6), 1275-1280. https://doi.org/10.1016/S0168-9452(03)00336-4