A small GTP-binding host protein is required for entry of powdery mildew fungus into epidermal cells of barley - Research portal of Justus Liebig University Giessen:

Journal article

A small GTP-binding host protein is required for entry of powdery mildew fungus into epidermal cells of barley

Authors list: Schultheiss, H; Dechert, C; Kogel, KH; Hückelhoven, R

Publication year: 2002

Pages: 1447-1454

Journal: Plant Physiology

Volume number: 128

Issue number: 4

ISSN: 0032-0889

Open access status: Green

DOI Link: https://doi.org/10.1104/pp.010805

Publisher: Oxford University Press

Abstract:
Small GTP-binding proteins such as those from the RAC family are cytosolic signal transduction proteins that often are involved in processing of extracellular stimuli. Plant RAC proteins are implicated in regulation of plant cell architecture, secondary wall formation, meristem signaling, and defense against pathogens. We isolated a RacB homolog from barley (Hordeum vulgare) to study its role in resistance to the barley powdery mildew fungus (Blumeria graminis f.sp. hordei). RacB was constitutively expressed in the barley epidermis and its expression level was not strongly influenced by inoculation with B. graminis. However, after biolistic bombardment of barley leaf segments with RacB-double-stranded RNA, sequence-specific RNA interference with RacB function inhibited fungal haustorium establishment in a cell-autonomous and genotype-specific manner. Mutants compromised in function of the Mlo wild-type gene and the Ror1 gene (genotype mlo5 ror1) that are moderately susceptible to B. graminis showed no alteration in powdery mildew resistance upon RacB-specific RNA interference. Thus, the phenotype, induced by RacB-specific RNA interference, was apparently dependent on the same processes as mlo5-mediated broad resistance, which is suppressed by ror1. We conclude that an RAC small GTP-binding protein is required for successful fungal haustorium establishment and that this function may be linked to MLO-associated functions.

Authors/Editors

- Prof. Dr. Karl-Heinz Kogel

Citation Styles

Harvard Citation style: Schultheiss, H., Dechert, C., Kogel, K. and Hückelhoven, R. (2002) A small GTP-binding host protein is required for entry of powdery mildew fungus into epidermal cells of barley, Plant Physiology, 128(4), pp. 1447-1454. https://doi.org/10.1104/pp.010805

APA Citation style: Schultheiss, H., Dechert, C., Kogel, K., & Hückelhoven, R. (2002). A small GTP-binding host protein is required for entry of powdery mildew fungus into epidermal cells of barley. Plant Physiology. 128(4), 1447-1454. https://doi.org/10.1104/pp.010805

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