Barley Mla and Rar mutants compromised in the hypersensitive cell death response against Blumeria graminis f.sp. hordei are modified in their ability to accumulate reactive oxygen intermediates at sites of fungal invasion - Research portal of Justus Liebig University Giessen:

Journal article

Barley Mla and Rar mutants compromised in the hypersensitive cell death response against Blumeria graminis f.sp. hordei are modified in their ability to accumulate reactive oxygen intermediates at sites of fungal invasion

Authors list: Hückelhoven, R; Fodor, J; Trujillo, M; Kogel, KH

Publication year: 2000

Pages: 16-24

Journal: Planta

Volume number: 212

Issue number: 1

ISSN: 0032-0935

DOI Link: https://doi.org/10.1007/s004250000385

Publisher: Springer

Abstract:
The pathogenesis-related accumulation of superoxide radical anions (O-2(.-)) and hydrogen peroxide (H2O2) was comparatively analyzed in a barley line (Hordeum vulgare L. cv Sultan-5) carrying the powdery mildew (Blumeria graminis f.sp. hordei, Speer, Bgh) resistance gene Mlal2, and in susceptible mutants defective in Mlal2 or in genes "required for Mlal2-specified disease resistance" (Rar1 and Rar2). In-situ localization of reactive oxygen intermediates was performed both by microscopic detection of azide-insensitive nitroblue tetrazolium (NBT) reduction or diaminobenzidine (DAB) polymerization, and by an NBT-DAB double-staining procedure. The Mlal2-mediated hypersensitive cell death occurred either in attacked epidermal cells or adjacent mesophyll cells of wild-type plants. Whole-cell H2O2 accumulation was detected in dying cells, while O-2(.-) emerged in adjacent cells. Importantly, all susceptible mutants lacked these reactions. An oxalate oxidase, which is known to generate H2O2 and has been implicated in barley resistance against the powdery mildew fungus, was not differentially expressed between the wild type and all mutants. The results demonstrate that the Rar1 and RaR2 gene products, which are control elements of R-gene-mediated programmed cell death, also control accumulation of reactive oxygen intermediates but not the pathogenesis-related expression of oxalate oxidase.

Authors/Editors

- Prof. Dr. Karl-Heinz Kogel

Citation Styles

Harvard Citation style: Hückelhoven, R., Fodor, J., Trujillo, M. and Kogel, K. (2000) Barley Mla and Rar mutants compromised in the hypersensitive cell death response against Blumeria graminis f.sp. hordei are modified in their ability to accumulate reactive oxygen intermediates at sites of fungal invasion, Planta, 212(1), pp. 16-24. https://doi.org/10.1007/s004250000385

APA Citation style: Hückelhoven, R., Fodor, J., Trujillo, M., & Kogel, K. (2000). Barley Mla and Rar mutants compromised in the hypersensitive cell death response against Blumeria graminis f.sp. hordei are modified in their ability to accumulate reactive oxygen intermediates at sites of fungal invasion. Planta. 212(1), 16-24. https://doi.org/10.1007/s004250000385

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