Journal article

Publication year: 2002

Pages: 458-460

Journal: Plant protection science

Volume number: 38

Issue number: SI 2

DOI Link: https://doi.org/10.17221/10523-PPS

Publisher: Czech Academy of Agricultural Science

Abstract: 

The role of reactive oxygen intermediate (ROI) accumulation in resistance and susceptibility of plants to parasitic fungi is still little understood. We examined the spatial and temporal occurrence of different ROIs in barley after inoculation with the biotrophic fungus Blumeria graminis f.sp. hordei (Bgh, barley powdery mildew fungus). Using histochemical analyses, we collected correlative data indicating that H₂O₂ and O₂^•– play different roles in background penetration resistance to Bgh. To study the role of O₂^•– in detail, we isolated barley cDNAs encoding a NADPH oxidase GP91PHOX homologue and a RACB homologue, which may be involved in NADPH oxidase activation. Interestingly, transient silencing of RACB or GP91PHOX via sequence-specific RNA interference enhanced penetration resistance of barley to Bgh. Together, data reveal rather a negative than a positive role of superoxide generation in background resistance of barley to Bgh.

Harvard Citation style: Hückelhoven, R., Trujillo, M., Dechert, C., Schultheiss, H. and Kogel, K. (2002) Functional studies on the role of reactive oxygen intermediates in the resistance of barley against powdery mildew, Plant protection science, 38(SI 2), pp. 458-460. https://doi.org/10.17221/10523-PPS

APA Citation style: Hückelhoven, R., Trujillo, M., Dechert, C., Schultheiss, H., & Kogel, K. (2002). Functional studies on the role of reactive oxygen intermediates in the resistance of barley against powdery mildew. Plant protection science. 38(SI 2), 458-460. https://doi.org/10.17221/10523-PPS