Journalartikel

Jahr der Veröffentlichung: 2006

Seiten: 3718-3726

Zeitschrift: Biophysical Journal

Bandnummer: 91

Heftnummer: 10

ISSN: 0006-3495

DOI Link: https://doi.org/10.1529/biophysj.106.086900

Verlag: Biophysical Society

Abstract: 
The glutamate transporter GLT-1 from Rattus norvegicus was expressed at high level in baby hamster kidney (BHK-21) cells by the Semliki Forest Virus expression system. We examined the expressed GLT-1 in the plasma membrane and found that the transporter accumulates in detergent-insoluble lipid-protein assemblies. Freeze-fracture, immunogold labeling, and electron microscopy revealed that GLT-1 forms similar to 200-nm protein-rich islands in the plasma membrane. Cholesterol depletion in living cells resulted in a dispersion of the GLT-1 islands, indicating that they are the result of lipid-protein rather than protein-protein interactions. Disruption of GLT-1 islands and dispersion of GLT-1 goes along with a reduction of the glutamate transport activity. Our direct visualization of lipid-protein islands in the plasma membrane of tissue culture cells suggests that the reported clustering of glutamate transporters and their cholesterol-dependent transport activity in cells is likewise connected to their association with cholesterol-rich microdomains in the plasma membrane.

Harvard-Zitierstil: Raunser, S., Haase, W., Franke, C., Eckert, G., Müller, W. and Kühlbrandt, W. (2006) Heterologously expressed GLT-1 associates in similar to 200-nm protein-lipid islands, Biophysical Journal, 91(10), pp. 3718-3726. https://doi.org/10.1529/biophysj.106.086900

APA-Zitierstil: Raunser, S., Haase, W., Franke, C., Eckert, G., Müller, W., & Kühlbrandt, W. (2006). Heterologously expressed GLT-1 associates in similar to 200-nm protein-lipid islands. Biophysical Journal. 91(10), 3718-3726. https://doi.org/10.1529/biophysj.106.086900