Journal article

Publication year: 2020

Pages: 344-350

Journal: Journal of Liquid Chromatography and Related Technologies

Volume number: 43

Issue number: 9-10

ISSN: 1082-6076

DOI Link: https://doi.org/10.1080/10826076.2020.1737542

Publisher: Taylor and Francis Group

Abstract: 
Aerial parts of Plectranthus amboinicus (Lour.) were collected in the Northern Mexico City for a comprehensive effect-directed profiling. Its hydrodistilled P. amboinicus essential oil (PAEO) was separated on HPTLC silica gel plates with n-hexane-ethyl acetate-ethanol, 95:3:2, followed by derivatization with the anisaldehyde - sulfuric acid reagent. The UV/Vis/FLD detection was expanded by a biological and biochemical detection. Eight different effect-directed assays were performed including the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay as well as biochemical assays for inhibition of acetylcholinesterase, alpha- and beta-glucosidases, alpha-amylase and tyrosinase as well as antimicrobial assays against Gram-negative Aliivibrio fischeri and Gram-positive Bacillus subtilis bacteria. The bioprofiling, subsequent direct analysis in real time mass spectrometry of the detected five bioactive compound zones and comparison with literature data resulted in the tentative assignment of caryophyllene oxide (hR(F) 20), alpha-humulene (hR(F) 26), carvacrol (hR(F) 40), methyl carvacrol ether (hR(F) 76) and caryophyllene (hR(F) 84). The antimicrobial activity against Gram-negative bacteria of PAEO was elicited by a mixture of the multi-potent compounds, whereas alpha-humulene was strongly acting against Gram-positive bacteria. The antioxidant effect was related to carvacrol and the inhibitory effect over AChE, tyrosinase and glucosidases to alpha-humulene.

Harvard Citation style: Banuelos-Hernandez, A., Azadniya, E., Ramirez Moreno, E. and Morlock, G. (2020) Bioprofiling of Mexican Plectranthus amboinicus (Lour.) essential oil via planar chromatography-effect-directed analysis combined with direct analysis in real time high-resolution mass spectrometry, Journal of Liquid Chromatography and Related Technologies, 43(9-10), pp. 344-350. https://doi.org/10.1080/10826076.2020.1737542

APA Citation style: Banuelos-Hernandez, A., Azadniya, E., Ramirez Moreno, E., & Morlock, G. (2020). Bioprofiling of Mexican Plectranthus amboinicus (Lour.) essential oil via planar chromatography-effect-directed analysis combined with direct analysis in real time high-resolution mass spectrometry. Journal of Liquid Chromatography and Related Technologies. 43(9-10), 344-350. https://doi.org/10.1080/10826076.2020.1737542