Journal article

Publication year: 2021

Journal: Journal of Proteomics

Volume number: 242

Issue number: 2021

DOI Link: https://doi.org/10.1016/j.jprot.2021.104255

Publisher: Elsevier

Abstract: 

Hepatic steatosis is a very common response to liver injury and often attributed to metabolic disorders. Prior studies have demonstrated the efficacy of a biotechnologically produced oyster mushroom (Pleurotus sajor-caju, PSC) in alleviating hepatic steatosis in obese Zucker rats. This study aims to elucidate molecular events underlying the anti-steatotic effects of PSC. Tandem mass tag (TMT) peptide labeling coupled with LC-MS/MS/MS was used to quantify and compare proteins in the livers of lean Zucker rats fed a control diet (LC), obese Zucker rats fed the same control diet (OC) and obese Zucker rats fed the control diet supplemented with 5% PSC (OPSC) for 4 weeks. Using this technique 3128 proteins could be quantified, out of which 108 were differentially abundant between the OPSC and OC group. Functional enrichment analysis of the up-regulated proteins showed that these proteins were mainly involved in metabolic processes, while the down-regulated proteins were involved in inflammatory processes. Results from proteomic analysis were successfully validated for two upregulated (carbonic anhydrase 3, regucalcin) and two down-regulated (cadherin-17, ceruloplasmin) proteins by means of immunoblotting.& nbsp; Significance: Valorization of low-grade agricultural waste by edible fungi, such as the mushroom Pleurotus sajorcaju (PSC), represents a promising strategy for the production of protein rich biomass since they boast of a unique enzyme system that has the ability to recover nutrients and energy from biodegradable waste. Herein, we describe the metabolic effects of PSC feeding using a combined quantitative proteomics and bioinformatics approach. In total, 108 proteins were identified to be regulated by PSC feeding in the liver of the obese rats. Complementary usage of a bioinformatics approach allowed us to decipher the mechanisms underlying the recently observed lipid-lowering and anti-inflammatory activity of PSC feeding in obese Zucker rats, namely a reduction of fatty acid synthesis, an improvement of hepatoprotective mechanisms and an enhancement of antiinflammatory effects.

Harvard Citation style: Maheshwari, G., Wen, G., Gessner, D., Ringseis, R., Lochnit, G., Eder, K., et al. (2021) Tandem mass tag-based proteomics for studying the effects of a biotechnologically produced oyster mushroom against hepatic steatosis in obese Zucker rats, Journal of Proteomics, 242(2021), Article 104255. https://doi.org/10.1016/j.jprot.2021.104255

APA Citation style: Maheshwari, G., Wen, G., Gessner, D., Ringseis, R., Lochnit, G., Eder, K., Zorn, H., & Timm, T. (2021). Tandem mass tag-based proteomics for studying the effects of a biotechnologically produced oyster mushroom against hepatic steatosis in obese Zucker rats. Journal of Proteomics. 242(2021), Article 104255. https://doi.org/10.1016/j.jprot.2021.104255