Journal article

Publication year: 2021

Pages: 6660-6672

Journal: Nucleic Acids Research

Volume number: 49

Issue number: 12

ISSN: 0305-1048

Open access status: Gold

DOI Link: https://doi.org/10.1093/nar/gkab468

Publisher: Oxford University Press

Abstract: 

Elucidating the structure of RNA and RNA ensembles is essential to understand biological functions. In this work, we explored the previously uncharted reactivity of bis-chloropiperidines (B-CePs) towards RNA. We characterized at the molecular level the different adducts induced by the fast reacting compound B-CeP 1 with RNA. Following an approach based on solution thermal melting coupled with ESI mass spectrometry (STHEM-ESI), we proved the ability of B-CePs to induce inter-molecular cross-links between guanines in double stranded RNA. These results open the possibility of using B-CePs as structural probes for investigating higher-order structures, such as the kissing loop complex established by the dimerization initiation site (DIS) of the HIV-1 genome. We confirmed the potential of B-CePs to reveal the identity of RNA structures involved in long-range interactions, expecting to benefit the characterization of samples that are not readily amenable to traditional high-resolution techniques, and thus promoting the elucidation of pertinent RNA systems associated with old and new diseases.

Harvard Citation style: Sosic, A., Göttlich, R., Fabris, D. and Gatto, B. (2021) B-CePs as cross-linking probes for the investigation of RNA higher-order structure, Nucleic Acids Research, 49(12), pp. 6660-6672. https://doi.org/10.1093/nar/gkab468

APA Citation style: Sosic, A., Göttlich, R., Fabris, D., & Gatto, B. (2021). B-CePs as cross-linking probes for the investigation of RNA higher-order structure. Nucleic Acids Research. 49(12), 6660-6672. https://doi.org/10.1093/nar/gkab468