Journal article

Publication year: 2021

Pages: 101565-

Journal: MethodsX

Volume number: 8

Open access status: Gold

DOI Link: https://doi.org/10.1016/j.mex.2021.101565

Publisher: Elsevier

Abstract: 
Miniaturization of biomedical and chemical research areas is performed using microfluidic techniques. Droplet-based microfluidic applications are of high interest for various applications, e.g., high-throughput screening assays. Many of them are based on simple water-in-oil (w/o) or oil-in-water (o/w) emulsions that are easily to produce. More complex assays based on separate compartments require the use of multiple emulsions, such as water-in-oil-in-water (w/o/w) or oil-in-water-in-oil (o/w/o) emulsions. In this study an easy, fast to establish method to generate agarose-solidified (w/w/o) double emulsions with similar to 55 mu m in diameter, in which both agarose-phases are not separated by a surfactant stabilized oil is described. An off-chip emulsion-breaking and washing step of the inner agarose droplets based on density gradient centrifugation was designed, offering new possibilities for high-throughput assays on picoliter scale. In brief, this paper reports:the protocol to generate agarose-solidified (w/w/o) double emulsions non-seperated by surfactant stabilized oil;an off-chip washing protocol of agarose-solidified emulsions based on density gradient centrifugation. (C) 2021 The Authors. Published by Elsevier B.V.

Harvard Citation style: Brinkmann, S., Oberpaul, M., Glaeser, J. and Schäberle, T. (2021) Two-step generation of monodisperse agarose-solidified double emulsions (w/w/o) excluding an inner oil barrier, MethodsX, 8, p. 101565. https://doi.org/10.1016/j.mex.2021.101565

APA Citation style: Brinkmann, S., Oberpaul, M., Glaeser, J., & Schäberle, T. (2021). Two-step generation of monodisperse agarose-solidified double emulsions (w/w/o) excluding an inner oil barrier. MethodsX. 8, 101565. https://doi.org/10.1016/j.mex.2021.101565