Identification, Characterization, and Synthesis of Natural Parasitic Cysteine Protease Inhibitors: Pentacitidins Are More Potent Falcitidin Analogues - Research portal of Justus Liebig University Giessen:

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Identification, Characterization, and Synthesis of Natural Parasitic Cysteine Protease Inhibitors: Pentacitidins Are More Potent Falcitidin Analogues

Authors list: Brinkmann, S; Semmler, S; Kersten, C; Patras, MA; Kurz, M; Fuchs, N; Hammerschmidt, SJ; Legac, J; Hammann, PE; Vilcinskas, A; Rosenthal, PJ; Schirmeister, T; Bauer, A; Schäberle, TF

Publication year: 2022

Journal: BioRxiv

DOI Link: https://doi.org/10.1101/2021.10.30.466580

Publisher: Cold Spring Harbor Laboratory

Abstract:

Protease inhibitors represent a promising therapeutic option for the treatment of parasitic diseases such as malaria and human African trypanosomiasis. Falcitidin was the first member of a new class of inhibitors of falcipain-2, a cysteine protease of the malaria parasite Plasmodium falciparum. Using a metabolomics dataset of 25 Chitinophaga strains for molecular networking enabled identification of over 30 natural analogs of falcitidin. Based on MS/MS spectra, they vary in their amino acid chain length, sequence, acyl residue, and C-terminal functionalization; therefore, they were grouped into the four falcitidin peptide families A-D. The isolation, characterization and absolute structure elucidation of two falcitidin-related pentapeptide aldehyde analogs by extensive MS/MS spectrometry and NMR spectroscopy in combination with advanced Marfey’s analysis was in agreement with the in silico analysis of the corresponding biosynthetic gene cluster. Total synthesis of chosen pentapeptide analogs followed by in vitro testing against a panel of proteases revealed selective parasitic cysteine protease inhibition and additionally low-micromolar inhibition of α-chymotrypsin. The pentapeptides investigated here showed superior inhibitory activity compared to falcitidin.

Authors/Editors

- Uni.-Prof. Dr. Till Friedrich Schäberle

Citation Styles

Harvard Citation style: Brinkmann, S., Semmler, S., Kersten, C., Patras, M., Kurz, M., Fuchs, N., et al. (2022) Identification, Characterization, and Synthesis of Natural Parasitic Cysteine Protease Inhibitors: Pentacitidins Are More Potent Falcitidin Analogues [Preprint]. BioRxiv, Article 2021.10.30.466580. https://doi.org/10.1101/2021.10.30.466580

APA Citation style: Brinkmann, S., Semmler, S., Kersten, C., Patras, M., Kurz, M., Fuchs, N., Hammerschmidt, S., Legac, J., Hammann, P., Vilcinskas, A., Rosenthal, P., Schirmeister, T., Bauer, A., & Schäberle, T. (2022). Identification, Characterization, and Synthesis of Natural Parasitic Cysteine Protease Inhibitors: Pentacitidins Are More Potent Falcitidin Analogues. BioRxiv. https://doi.org/10.1101/2021.10.30.466580