Contribution in an anthology

Appeared in: RNA Tagging

Editor list: Heinlein, M.

Publication year: 2020

Pages: 227-238

ISBN: 978-1-0716-0711-4

eISBN: 978-1-0716-0712-1

DOI Link: https://doi.org/10.1007/978-1-0716-0712-1_13

Title of series: Methods in Molecular Biology

Number in series: 2166

Abstract: 

Double-stranded RNA (dsRNA) plays an essential role in many biological processes and has a great potential for agronomic applications in disease and pest control. A simple and effective method to monitor dsRNA uptake by fungi is crucial for the use of dsRNA as alternative fungicide. The protocol reported in this chapter describes an efficient method to detect and localize labeled dsRNA in fungal hyphae. We use the fungal Verticillium longisporum, a fungal plant pathogen that commonly infects rapeseed and other Brassica species, to explain the procedure, though we have validated the method in a broad spectrum of fungi. Hereafter we elucidate step-by-step the production, fluorescence labeling, as well as detection of dsRNA via fluorescence microscopy in fungal mycelium.

Harvard Citation style: Galli, M., Imani, J. and Kogel, K. (2020) Labeling of dsRNA for Fungal Uptake Detection Analysis, in Heinlein, M. (ed.) RNA Tagging. New York, NY: Humana, pp. 227-238. https://doi.org/10.1007/978-1-0716-0712-1_13

APA Citation style: Galli, M., Imani, J., & Kogel, K. (2020). Labeling of dsRNA for Fungal Uptake Detection Analysis. In Heinlein, M. (Ed.), RNA Tagging (pp. 227-238). Humana. https://doi.org/10.1007/978-1-0716-0712-1_13