Journalartikel

Jahr der Veröffentlichung: 2021

Seiten: 36-46

Zeitschrift: Methods

Bandnummer: 196

ISSN: 1046-2023

Open Access Status: Hybrid

DOI Link: https://doi.org/10.1016/j.ymeth.2021.04.014

Verlag: Elsevier

Abstract: 
Circular RNAs (circRNAs) have been studied extensively in the last few years, uncovering functional roles in a diverse range of cell types and organisms. As shown for a few cases, these functions may be mediated by trans- acting factors, in particular RNA-binding proteins (RBPs). However, the specific interaction partners for most circRNAs remain unknown. This is mainly due to technical difficulties in their identification and in differentiating between interactors of circRNAs and their linear counterparts. Here we review the currently used methodology to systematically study circRNA-protein complexes (circRNPs), focusing either on a specific RNA or protein, both on the gene-specific or global level, and discuss advantages and challenges of the available approaches.

Harvard-Zitierstil: Ulshöfer, C., Pfafenrot, C., Bindereif, A. and Schneider, T. (2021) Methods to study circRNA-protein interactions, Methods, 196, pp. 36-46. https://doi.org/10.1016/j.ymeth.2021.04.014

APA-Zitierstil: Ulshöfer, C., Pfafenrot, C., Bindereif, A., & Schneider, T. (2021). Methods to study circRNA-protein interactions. Methods. 196, 36-46. https://doi.org/10.1016/j.ymeth.2021.04.014