Journal article

Publication year: 2020

Pages: 12493-12502

Journal: Journal of Agricultural and Food Chemistry

Volume number: 68

Issue number: 44

ISSN: 0021-8561

DOI Link: https://doi.org/10.1021/acs.jafc.0c05163

Publisher: American Chemical Society

Abstract: 
Liquid chromatography coupled with high resolution mass spectrometry (LC-HRMS) complements standard triple-quadrupole mass spectrometry in veterinary drug residue control. LC-HRMS offers the opportunity for nontargeted screening for metabolites and biomarkers representing metabolic changes. In this work, the feasibility of a nontargeted metabolomics approach based on LC-HRMS data (LC-Q-Orbitrap and LC-Q-TOF) to distinguish between porcine muscle tissue from infected animals and from healthy animals is demonstrated. The differences arise from various compounds associated with metabolic changes in infected animals. Two new biomarker candidates have been identified: tripeptide prolyphenylalanylglycine and a lysophosphatidylcholine derivative. For the first time, a bivariate data analysis procedure is described that may be used to evaluate whether the presence of antibiotic residues points to a therapeutic application or may be the result of a contamination during sampling and/or analysis.

Harvard Citation style: Liesenfeld, S., Steliopoulos, P. and Hamscher, G. (2020) Comprehensive Metabolomics Analysis of Nontargeted LC-HRMS Data Provides Valuable Insights Regarding the Origin of Veterinary Drug Residues, Journal of Agricultural and Food Chemistry, 68(44), pp. 12493-12502. https://doi.org/10.1021/acs.jafc.0c05163

APA Citation style: Liesenfeld, S., Steliopoulos, P., & Hamscher, G. (2020). Comprehensive Metabolomics Analysis of Nontargeted LC-HRMS Data Provides Valuable Insights Regarding the Origin of Veterinary Drug Residues. Journal of Agricultural and Food Chemistry. 68(44), 12493-12502. https://doi.org/10.1021/acs.jafc.0c05163