Journal article

Publication year: 2019

Pages: 1812-1820

Journal: Cell Reports

Volume number: 29

Issue number: 7

ISSN: 2211-1247

Open access status: Gold

DOI Link: https://doi.org/10.1016/j.celrep.2019.10.025

Publisher: Cell Press

Abstract: 
The DUX4 transcription factor is briefly expressed in the early cleavage-stage embryo, where it induces an early wave of zygotic gene transcription, whereas its mis-expression in skeletal muscle causes the muscular dystrophy facioscapulohumeral dystrophy (FSHD). Here, we show that DUX4 induces the expression of the histone variants H3.X and H3.Y. We have used a myoblast cell line with doxycycline-inducible DUX4 to show that these histone variants are incorporated throughout the body of DUX4-induced genes. Following a brief pulse of DUX4, these histones contribute to greater perdurance and to enhanced re-activation of DUX4 target gene expression. These findings provide a model for H3.X/Y as a chromatin mechanism that facilitates the expression of DUX4 target genes subsequent to a brief pulse of DUX4 expression.

Harvard Citation style: Resnick, R., Wong, C., Hamm, D., Bennett, S., Skene, P., Hake, S., et al. (2019) DUX4-Induced Histone Variants H3.X and H3.Y Mark DUX4 Target Genes for Expression, Cell Reports, 29(7), pp. 1812-1820. https://doi.org/10.1016/j.celrep.2019.10.025

APA Citation style: Resnick, R., Wong, C., Hamm, D., Bennett, S., Skene, P., Hake, S., Henikoff, S., van der Maarel, S., & Tapscott, S. (2019). DUX4-Induced Histone Variants H3.X and H3.Y Mark DUX4 Target Genes for Expression. Cell Reports. 29(7), 1812-1820. https://doi.org/10.1016/j.celrep.2019.10.025