Journalartikel

Jahr der Veröffentlichung: 2022

Seiten: 2235-2250

Zeitschrift: Analytical and Bioanalytical Chemistry

Bandnummer: 414

Heftnummer: 6

ISSN: 1618-2642

eISSN: 1618-2650

Open Access Status: Hybrid

DOI Link: https://doi.org/10.1007/s00216-021-03860-0

Verlag: Springer

Abstract: 
Peroxisomes are versatile single membrane-enclosed cytoplasmic organelles, involved in reactive oxygen species (ROS) and lipid metabolism and diverse other metabolic processes. Peroxisomal disorders result from mutations in Pex genes-encoded proteins named peroxins (PEX proteins) and single peroxisomal enzyme deficiencies. The PEX11 protein family (alpha, beta, and gamma isoforms) plays an important role in peroxisomal proliferation and fission. However, their specific functions and the metabolic impact caused by their deficiencies have not been precisely characterized. To understand the systemic molecular alterations caused by peroxisomal defects, here we utilized untreated peroxisomal biogenesis factor 11 alpha knockout (Pex11 alpha KO) mouse model and performed serial relative-quantitative lipidomic, metabolomic, and proteomic analyses of serum, liver, and heart tissue homogenates. We demonstrated significant specific changes in the abundances of multiple lipid species, polar metabolites, and proteins and dysregulated metabolic pathways in distinct biological specimens of the Pex11 alpha KO adult mice in comparison to the wild type (WT) controls. Overall, the present study reports comprehensive semi-quantitative molecular omics information of the Pex11 alpha KO mice, which might serve in the future as a reference for a better understanding of the roles of Pex11 alpha and underlying pathophysiological mechanisms of peroxisomal biogenesis disorders.

Harvard-Zitierstil: Garikapati, V., Colasante, C., Baumgart-Vogt, E. and Spengler, B. (2022) Sequential lipidomic, metabolomic, and proteomic analyses of serum, liver, and heart tissue specimens from peroxisomal biogenesis factor 11α knockout mice, Analytical and Bioanalytical Chemistry, 414(6), pp. 2235-2250. https://doi.org/10.1007/s00216-021-03860-0

APA-Zitierstil: Garikapati, V., Colasante, C., Baumgart-Vogt, E., & Spengler, B. (2022). Sequential lipidomic, metabolomic, and proteomic analyses of serum, liver, and heart tissue specimens from peroxisomal biogenesis factor 11α knockout mice. Analytical and Bioanalytical Chemistry. 414(6), 2235-2250. https://doi.org/10.1007/s00216-021-03860-0