Journal article

Atmospheric-Pressure MALDI Mass Spectrometry Imaging at 213 nm Laser Wavelength


Authors listHeiles, S; Kompauer, M; Müller, MA; Spengler, B

Publication year2020

Pages326-335

JournalJournal of The American Society for Mass Spectrometry

Volume number31

Issue number2

ISSN1044-0305

eISSN1879-1123

DOI Linkhttps://doi.org/10.1021/jasms.9b00052

PublisherAmerican Chemical Society


Abstract
First results for a new atmospheric-pressure matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging source operating at 213 nm laser wavelength are presented. The activation of analytes in the 213 nm MALDI process at atmospheric pressure was evaluated and compared to results for 337 nm MALDI and electrospray ionization using thermometer molecules. Different sample preparation techniques for nicotinic acid, the matrix with the highest ionization efficiency at 213 nm of all tested matrices, were evaluated and optimized to obtain small crystal sizes, homogenous matrix layer sample coverage, and high ion signal gains. Mass spectrometry imaging experiments of phospholipids in mouse tissue sections in positive- and negative-ion mode with different lateral resolutions and the corresponding pre-/post-mass spectrometry imaging workflows are presented. The use of custom-made objective lenses resulted in sample ablation spot diameters of on average 2.9 mu m, allowing mass spectrometry imaging experiments to be performed with 3 mu m pixel size without oversampling. The ion source was coupled to an orbital trapping mass spectrometer offering high mass resolution (>100.000), high mass accuracy (<= +/- 2 ppm), and high sensitivity (single pixel on-tissue tandem MS from 6.6 mu m(2) ablation area). The newly developed 213 nm atmospheric-pressure MALDI source combines the high mass resolution and high mass accuracy performance characteristics of orbital trapping mass spectrometers with high lateral resolution (pixel size similar to 3 mu m) mass spectrometry imaging.



Citation Styles

Harvard Citation styleHeiles, S., Kompauer, M., Müller, M. and Spengler, B. (2020) Atmospheric-Pressure MALDI Mass Spectrometry Imaging at 213 nm Laser Wavelength, Journal of The American Society for Mass Spectrometry, 31(2), pp. 326-335. https://doi.org/10.1021/jasms.9b00052

APA Citation styleHeiles, S., Kompauer, M., Müller, M., & Spengler, B. (2020). Atmospheric-Pressure MALDI Mass Spectrometry Imaging at 213 nm Laser Wavelength. Journal of The American Society for Mass Spectrometry. 31(2), 326-335. https://doi.org/10.1021/jasms.9b00052


Last updated on 2025-21-05 at 16:48