Journalartikel

Jahr der Veröffentlichung: 2019

Seiten: 20-27

Zeitschrift: Rapid Communications in Mass Spectrometry

Bandnummer: 33

Heftnummer: S1

ISSN: 0951-4198

eISSN: 1097-0231

Open Access Status: Bronze

DOI Link: https://doi.org/10.1002/rcm.8255

Verlag: Wiley

Abstract: 
The venom produced by snakes contains complex mixtures of pharmacologically active proteins and peptides which play a crucial role in the pathophysiology of snakebite diseases. The deep understanding of venom proteomes can help to improve the treatment of this "neglected tropical disease" (as expressed by the World Health Organization [WHO]) and to develop new drugs. The most widely used technique for venom analysis is liquid chromatography/tandem mass spectrometry (LC/MS/MS)-based bottom-up (BU) proteomics. Considering the fact that multiple multi-locus gene families encode snake venom proteins, the major challenge for the BU proteomics is the limited sequence coverage and also the "protein inference problem" which result in a loss of information for the identification and characterization of toxin proteoforms (genetic variation, alternative mRNA splicing, single nucleotide polymorphism [SNP] and post-translational modifications [PTMs]). In contrast, intact protein measurements with top-down (TD) MS strategies cover almost complete protein sequences, and prove the ability to identify venom proteoforms and to localize their modifications and sequence variations.

Harvard-Zitierstil: Ghezellou, P., Garikapati, V., Kazemi, S., Strupat, K., Ghassempour, A. and Spengler, B. (2019) A perspective view of top-down proteomics in snake venom research, Rapid Communications in Mass Spectrometry, 33(S1), pp. 20-27. https://doi.org/10.1002/rcm.8255

APA-Zitierstil: Ghezellou, P., Garikapati, V., Kazemi, S., Strupat, K., Ghassempour, A., & Spengler, B. (2019). A perspective view of top-down proteomics in snake venom research. Rapid Communications in Mass Spectrometry. 33(S1), 20-27. https://doi.org/10.1002/rcm.8255