Journalartikel

Jahr der Veröffentlichung: 2019

Seiten: 4394-4406

Zeitschrift: ACS Chemical Neuroscience

Bandnummer: 10

Heftnummer: 10

ISSN: 1948-7193

DOI Link: https://doi.org/10.1021/acschemneuro.9b00435

Verlag: American Chemical Society

Abstract: 
Lipids, including omega-3 polyunsaturated fatty acids (n-3-PUFAs), modulate brain-intrinsic inflammation during systemic inflammation. The vascular organ of the lamina terminalis (OVLT) is a brain structure important for immune-to-brain communication. We, therefore, aimed to profile the distribution of several lipids (e.g., phosphatidyl-choline/ethanolamine, PC/PE), including n-3-PUFA-carrying lipids (esterified in phospholipids), in the OVLT during systemic lipopolysaccharide(LPS)-induced inflammation. We injected wild type and endogenously n-3-PUFA producing fat-1 transgenic FAT-1 WT mice with LPS (i.p., 2.5 mg/kg) or PBS. Brain samples were analyzed using immunohistochemistry and high-resolution atmospheric-pressure scanning microprobe matrix-assisted laser desorption/ionization orbital trapping mass spectrometry imaging (APSMALDI-MSI) for spatial resolution of lipids. Depending on genotype and treatment, several distinct distribution patterns were observed for lipids [e.g., lyso(L)PC (16:0)/(18:0)] proposed to be involved in inflammation. The distribution patterns ranged from being homogeneously disseminated [LPC (18:1)], absent/reduced signaling within the OVLT relative to adjacent preoptic tissue [PE (38:6)], either treatment- and genotype-dependent or independent low signal intensities [LPC (18:0)], treatment- and genotype-dependent [PC 38:6)] or independent accumulation in the OVLT [PC (38:7)], and accumulation in commissures, e.g., nerve fibers like the optic nerve [LPE (18:1)]. Overall, screening of lipid distribution patterns revealed distinct inflammation-induced changes in the OVLT, highlighting the prominent role of lipid metabolism in brain inflammation. Moreover, known and novel candidates for brain inflammation and immune-to-brain communication were detected specifically within this pivotal brain structure, a window between the periphery and the brain. The biological significance of these newly identified lipids abundant in the OVLT and the adjacent preoptic area remains to be further analyzed.

Harvard-Zitierstil: Bredehöft, J., Bhandari, D., Pflieger, F., Schulz, S., Kang, J., Layé, S., et al. (2019) Visualizing and Profiling Lipids in the OVLT of Fat-1 and Wild Type Mouse Brains during LPS-Induced Systemic Inflammation Using AP-SMALDI MSI, ACS Chemical Neuroscience, 10(10), pp. 4394-4406. https://doi.org/10.1021/acschemneuro.9b00435

APA-Zitierstil: Bredehöft, J., Bhandari, D., Pflieger, F., Schulz, S., Kang, J., Layé, S., Roth, J., Gerstberger, R., Mayer, K., Spengler, B., & Rummel, C. (2019). Visualizing and Profiling Lipids in the OVLT of Fat-1 and Wild Type Mouse Brains during LPS-Induced Systemic Inflammation Using AP-SMALDI MSI. ACS Chemical Neuroscience. 10(10), 4394-4406. https://doi.org/10.1021/acschemneuro.9b00435