Journal article

Publication year: 2021

Pages: 37-

Journal: AMB Express

Volume number: 11

Issue number: 1

ISSN: 2191-0855

Open access status: Gold

DOI Link: https://doi.org/10.1186/s13568-021-01195-8

Publisher: SpringerOpen

Abstract: 
The basidiomycetous lipoxygenase Lox1 from Cyclocybe aegerita catalyzes the oxygenation of polyunsaturated fatty acids (PUFAs) with a high preference towards the C18-PUFA linoleic acid (C18:2 (omega-6)). In contrast, longer PUFAs, generally not present in the fungal cell such as eicosatrienoic acid (C20:3(omega-3)) and docosatrienoic acid (C22:3 (omega-3)), are converted with drastically lower activities. With site-directed mutagenesis, we were able to create two variants with enhanced activities towards longer chain PUFAs. The W330L variant showed a similar to 20 % increased specific activity towards C20:3(omega-3), while a similar to 2.5-fold increased activity against C22:3 (omega-3) was accomplished by the V581 variant.

Harvard Citation style: Karrer, D., Gand, M. and Rühl, M. (2021) Engineering a lipoxygenase from cyclocybe aegerita towards long chain polyunsaturated fatty acids, AMB Express, 11(1), Article 37. p. 37. https://doi.org/10.1186/s13568-021-01195-8

APA Citation style: Karrer, D., Gand, M., & Rühl, M. (2021). Engineering a lipoxygenase from cyclocybe aegerita towards long chain polyunsaturated fatty acids. AMB Express. 11(1), Article 37, 37. https://doi.org/10.1186/s13568-021-01195-8