Journalartikel

Jahr der Veröffentlichung: 2013

Seiten: 3010-3021

Zeitschrift: Nucleic Acids Research

Bandnummer: 41

Heftnummer: 5

ISSN: 0305-1048

eISSN: 1362-4962

Open Access Status: Gold

DOI Link: https://doi.org/10.1093/nar/gkt035

Verlag: Oxford University Press

Abstract: 
The heterogeneous collection of nucleosome remodelling and deacetylation (NuRD) complexes can be grouped into the MBD2- or MBD3-containing complexes MBD2-NuRD and MBD3-NuRD. MBD2 is known to bind to methylated CpG sequences in vitro in contrast to MBD3. Although functional differences have been described, a direct comparison of MBD2 and MBD3 in respect to genome-wide binding and function has been lacking. Here, we show that MBD2-NuRD, in contrast to MBD3-NuRD, converts open chromatin with euchromatic histone modifications into tightly compacted chromatin with repressive histone marks. Genome-wide, a strong enrichment for MBD2 at methylated CpG sequences is found, whereas CpGs bound by MBD3 are devoid of methylation. MBD2-bound genes are generally lower expressed as compared with MBD3-bound genes. When depleting cells for MBD2, the MBD2-bound genes increase their activity, whereas MBD2 plus MBD3-bound genes reduce their activity. Most strikingly, MBD3 is enriched at active promoters, whereas MBD2 is bound at methylated promoters and enriched at exon sequences of active genes.

Harvard-Zitierstil: Günther, K., Rust, M., Leers, J., Boettger, T., Scharfe, M., Jarek, M., et al. (2013) Differential roles for MBD2 and MBD3 at methylated CpG islands, active promoters and binding to exon sequences , Nucleic Acids Research, 41(5), pp. 3010-3021. https://doi.org/10.1093/nar/gkt035

APA-Zitierstil: Günther, K., Rust, M., Leers, J., Boettger, T., Scharfe, M., Jarek, M., Bartkuhn, M., & Renkawitz, R. (2013). Differential roles for MBD2 and MBD3 at methylated CpG islands, active promoters and binding to exon sequences . Nucleic Acids Research. 41(5), 3010-3021. https://doi.org/10.1093/nar/gkt035