Journal article
Authors list: Mohan, M; Bartkuhn, M; Herold, M; Philippen, A; Heinl, N; Bardenhagen, I; Leers, J; White, RAH; Renkawitz-Pohl, R; Saumweber, H; Renkawitz, R
Publication year: 2007
Pages: 4203-4214
Journal: The EMBO Journal
Volume number: 26
Issue number: 19
ISSN: 0261-4189
eISSN: 1460-2075
Open access status: Green
DOI Link: https://doi.org/10.1038/sj.emboj.7601851
Publisher: EMBO Press
Abstract:
Insulator sequences guide the function of distantly located enhancer elements to the appropriate target genes by blocking inappropriate interactions. In Drosophila, five different insulator binding proteins have been identified, Zw5, BEAF-32, GAGA factor, Su(Hw) and dCTCF. Only dCTCF has a known conserved counterpart in vertebrates. Here we find that the structurally related factors dCTCF and Su(Hw) have distinct binding targets. In contrast, the Su(Hw) interacting factor CP190 largely overlapped with dCTCF binding sites and interacts with dCTCF. Binding of dCTCF to targets requires CP190 in many cases, whereas others are independent of CP190. Analysis of the bithorax complex revealed that six of the borders between the parasegment specific regulatory domains are bound by dCTCF and by CP190 in vivo. dCTCF null mutations affect expression of Abdominal-B, cause pharate lethality and a homeotic phenotype. A short pulse of dCTCF expression during larval development rescues the dCTCF loss of function phenotype. Overall, we demonstrate the importance of dCTCF in fly development and in the regulation of abdominal segmentation.
Citation Styles
Harvard Citation style: Mohan, M., Bartkuhn, M., Herold, M., Philippen, A., Heinl, N., Bardenhagen, I., et al. (2007) The Drosophila insulator proteins CTCF and CP190 link enhancer blocking to body patterning, The EMBO Journal, 26(19), pp. 4203-4214. https://doi.org/10.1038/sj.emboj.7601851
APA Citation style: Mohan, M., Bartkuhn, M., Herold, M., Philippen, A., Heinl, N., Bardenhagen, I., Leers, J., White, R., Renkawitz-Pohl, R., Saumweber, H., & Renkawitz, R. (2007). The Drosophila insulator proteins CTCF and CP190 link enhancer blocking to body patterning. The EMBO Journal. 26(19), 4203-4214. https://doi.org/10.1038/sj.emboj.7601851
