Journalartikel

Jahr der Veröffentlichung: 2018

Seiten: 1786-1793

Zeitschrift: Ecology and Evolution

Bandnummer: 8

Heftnummer: 3

ISSN: 2045-7758

DOI Link: https://doi.org/10.1002/ece3.3687

Verlag: Wiley

Abstract: 
Properly designed (randomized and/or balanced) experiments are standard in ecological research. Molecular methods are increasingly used in ecology, but studies generally do not report the detailed design of sample processing in the laboratory. This may strongly influence the interpretability of results if the laboratory procedures do not account for the confounding effects of unexpected laboratory events. We demonstrate this with a simple experiment where unexpected differences in laboratory processing of samples would have biased results if randomization in DNA extraction and PCR steps do not provide safeguards. We emphasize the need for proper experimental design and reporting of the laboratory phase of molecular ecology research to ensure the reliability and interpretability of results.

Harvard-Zitierstil: Bálint, M., Márton, O., Schatz, M., Düring, R. and Grossart, H. (2018) Proper experimental design requires randomization/balancing of molecular ecology experiments, Ecology and Evolution, 8(3), pp. 1786-1793. https://doi.org/10.1002/ece3.3687

APA-Zitierstil: Bálint, M., Márton, O., Schatz, M., Düring, R., & Grossart, H. (2018). Proper experimental design requires randomization/balancing of molecular ecology experiments. Ecology and Evolution. 8(3), 1786-1793. https://doi.org/10.1002/ece3.3687