Journalartikel

Jahr der Veröffentlichung: 2021

Zeitschrift: Molecular Nutrition & Food Research

Bandnummer: 65

Heftnummer: 16

ISSN: 1613-4125

eISSN: 1613-4133

Open Access Status: Hybrid

DOI Link: https://doi.org/10.1002/mnfr.202100045

Verlag: Wiley

Abstract: 

Scope
To further examine the role of the human milk oligosaccharide 2'fucosyllactose (2 ' FL) and fucose (Fuc) in cognition. Using C-13-labeled 2'FL,thestudy previously showed in mice that C-13-enrichment of the brain is not caused by C-13(1)-2 ' FL itself, but rather by microbial metabolites. Here, the study applies C-13(1)-Fuc in the same mouse model to investigate its uptake into the brain.

Methods and Results
Mice received C-13(1)-Fuc via oral gavage (2 mmol C-13(1)-Fuc/kg(-1) body weight) or intravenously (0.4 mmol/kg(-1) body weight). C-13-enrichment is measured in organs, including various brain regions, biological fluids and excrements. By EA-IRMS, the study observes an early rise of C-13-enrichment in plasma, 30 min after oral dosing. However, C-13-enrichment in the brain does not occur until 3-5 h post-dosing, when the C-13-Fuc bolus has already reached the lower gut. Therefore, the researcher assume that C-13-Fuc is absorbed in the upper small intestine but cannot cross the blood-brain barrier which is also observed after intravenous application of C-13(1)-Fuc.

Conclusions
Late C-13-enrichment in the rodent brain may be derived from C-13(1)-Fuc metabolites derived from bacterial fermentation. The precise role that Fuc or 2 ' FL metabolites might play in gut-brain communication needs to be investigated in further studies.

Harvard-Zitierstil: Rudloff, S., Kuntz, S., Borsch, C., Vazquez, E., Buck, R., Reutzel, M., et al. (2021) Fucose as a Cleavage Product of 2’Fucosyllactose Does Not Cross the Blood-Brain Barrier in Mice, Molecular Nutrition & Food Research, 65(16), Article 2100045. https://doi.org/10.1002/mnfr.202100045

APA-Zitierstil: Rudloff, S., Kuntz, S., Borsch, C., Vazquez, E., Buck, R., Reutzel, M., Eckert, G., & Kunz, C. (2021). Fucose as a Cleavage Product of 2’Fucosyllactose Does Not Cross the Blood-Brain Barrier in Mice. Molecular Nutrition & Food Research. 65(16), Article 2100045. https://doi.org/10.1002/mnfr.202100045