Journal article

Publication year: 2019

Pages: 2928-2935

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 69

Issue number: 9

ISSN: 1466-5026

eISSN: 1466-5034

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijsem.0.003580

Publisher: Microbiology Society

Abstract: 
Three Gram-stain-positive, rod-to-coccoid-shaped, catalase-positive and non-motile bacterial strains isolated from the choanae of a Northern bald ibis, designated strains 200CH(T), W81(T) and 812CH(T), respectively, were subjected to comprehensive taxonomic characterization. The three strains were oxidase-negative. The 16S rRNA gene sequence of 200CH(T) showed highest similarities to Corynebacterium epidermidicanis 4101 (96.7 %) followed by Corynebacterium argentoratense DSM 44202(T), Corynebacterium ulcerans NCTC 79101 and Corynebacterium pseudotuberculosis CIP 1029681 (each 96.3 %). Strains W81 and 812CH(T) both showed highest 16S rRNA gene sequence similarities to Corynebacterium pelargi 136/3(T) (98.0 and 99.9 %, respectively). Comparison of the partial housekeeping gene sequence of fusA showed higher sequence similarities of 812CH(T) to C. pelargi (95.8 %) than W8(T) (90.9 %) which was also confirmed by corresponding amino acid sequences. In both, fusA gene and corresponding protein sequence strain 200CH(T) showed low sequence similarities to C. epidermidicanis 410(T) (81.6 and 87.4 %, respectively). Strains 812CH1 and W81 had 76.7 % ANI similarity to each other and 88.2 and 76.4 % to C. pelargi 136/31, respectively. In sitico DNA DNA hybridization values for 812CH(T) and W81 were 22.1 % among the two strains and 35.3 and 21.7% to C. pelargi 136/31, respectively. These data not only demonstrate that strain W81 is a representative of a novel species, but despite the high 16S rRNA gene sequence similarity to C. pelargi, strain 812CH(T) is also a representative of another novel species. All three strains possessed corynemycolic acids and contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan. The two strains, 200CH(T) and W8(T), are distinguished from each other and established Corynebacterium species phylogenetically and phenotypically. In conclusion, three novel species of the genus Corynebacterium are proposed, namely Corynebacterium pseudopelargi 812CH1 (=LMG 306271=CCM 88321), Corynebacterium choanae 200CH (=LMG 30628(T)=CCM 8831(T)) and Corynebacterium gerontici W81 (=LMG 306291=CCM 88331), respectively.

Harvard Citation style: Busse, H., Kleinhagauer, T., GLaeser, S., Spergser, J., Kämpfer, P. and Rückert, C. (2019) Classification of three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita): proposal of Corynebacterium choanae sp. nov., Corynebacterium pseudopelargi sp. nov., and Corynebacterium gerontici sp. nov., International Journal of Systematic and Evolutionary Microbiology, 69(9), pp. 2928-2935. https://doi.org/10.1099/ijsem.0.003580

APA Citation style: Busse, H., Kleinhagauer, T., GLaeser, S., Spergser, J., Kämpfer, P., & Rückert, C. (2019). Classification of three corynebacterial strains isolated from the Northern Bald Ibis (Geronticus eremita): proposal of Corynebacterium choanae sp. nov., Corynebacterium pseudopelargi sp. nov., and Corynebacterium gerontici sp. nov.. International Journal of Systematic and Evolutionary Microbiology. 69(9), 2928-2935. https://doi.org/10.1099/ijsem.0.003580