Journal article

Publication year: 2020

Pages: 2648-2656

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 70

Issue number: 4

ISSN: 1466-5026

eISSN: 1466-5034

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijsem.0.004086

Publisher: Microbiology Society

Abstract: 
From a phlegmon in a dog an aerobic and facultatively anaerobic, indole-, oxidase- and catalase-negative, non-motile bacterium was isolated in 2019 in Germany that stained Gram-negative and showed a pleomorphic, rod-shaped, non-spore-forming appearance. Based on the results of 16S rRNA gene sequence analyses, strain IHIT1603-19(T) was assigned to the genus Streptobacillus with sequence similarities of 98.6, 98.0, 97.9, 97.1 and 94.4% to the type strains of Streptobacillus felis, Streptobacillus notomytis, Streptobacillus ratti, Streptobacillus moniliformis and Streptobacillus hongkongensis, respectively. Strain IHIT1603-19(T) could also clearly be differentiated from other Streptobacillus species by rpoB, groEL and recA gene, nucleotide and amino acid sequence analyses as well as by core genome phylogeny. Regarding DNA-DNA relatedness, strain IHIT1603-19(T) demonstrated an average nucleotide identity of 83.00 and 82.28% compared to S. fells 131000547(T) and S. moniliformis DSM 12112(T), respectively. Chemotaxonomic and physiological data of strain IHIT1603-19(T) were in congruence with other closely related members of the family Leptotrichiaceae, represented by highly similar enzyme profiles and fatty acid patterns. MALDI-TOF MS analysis also proved suitable in unequivocally discriminating strain INIT1603-19(T) from all currently described taxa of the genus Streptobacillus. On the basis of these data, we propose the novel species Streptobacillus canis sp. nov. with the type strain IHIT1603-19(T) (=DSM 110501(T)=CCUG 74118(T)=CIP 111795(T)). The G+C content of the DNA of the type strain is 26.6mol%, genome size is 1.60 Mbp.

Harvard Citation style: Eisenberg, T., Heydel, C., Prenger-Berninghoff, E., Fawzy, A., Kling, U., Akimkin, V., et al. (2020) Streptobacillus canis sp. nov. isolated from a dog, International Journal of Systematic and Evolutionary Microbiology, 70(4), pp. 2648-2656. https://doi.org/10.1099/ijsem.0.004086

APA Citation style: Eisenberg, T., Heydel, C., Prenger-Berninghoff, E., Fawzy, A., Kling, U., Akimkin, V., Semmler, T., Mühldorfer, K., Kämpfer, P., Blom, J., & Ewers, C. (2020). Streptobacillus canis sp. nov. isolated from a dog. International Journal of Systematic and Evolutionary Microbiology. 70(4), 2648-2656. https://doi.org/10.1099/ijsem.0.004086