Journalartikel

Jahr der Veröffentlichung: 2020

Seiten: 4105-4110

Zeitschrift: International Journal of Systematic and Evolutionary Microbiology

Bandnummer: 70

Heftnummer: 7

ISSN: 1466-5026

eISSN: 1466-5034

Open Access Status: Bronze

DOI Link: https://doi.org/10.1099/ijsem.0.004230

Verlag: Microbiology Society

Abstract: 
A polyphasic taxonomic study was performed on an unidentified Arcanobacterium-like Gram-stain-positive bacterium designated strain C605018/01/1(T) isolated from a milk sample collected from the udder of a cow at post mortem. Comparative 16S rRNA gene sequencing showed that the bacterium belonged to the genus Arcanobacterium and was most closely related to the type strain of Arcanobacterium pluranimalium (99.76 %); sequence similarities to all other Arcanobacterium species were below 97 %. The wet-lab DNA-DNA hybridization values among strain C605018/01/1(T) and A. pluranimalium DSM 13483. were low, 16.9% (reciprocal, 49.8 %). Pertaining to the whole genome sequence with a total length of 2.02 Mb and 1654 protein counts, the novel strain C605018/01/01(T) displayed a G+C content of 51.6 % mol%. The presence of the major menaquinone MK-9(H 4) supported the affiliation of this strain to the genus Arcanobacterium. The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol-mannoside and unidentified glycolipid and aminophospholipids. Based on these results it is proposed that strain C605018/01/1(T) should be classified as representing a novel species, Arcanbacterium bovis sp. nov. The type strain C605018/01/1(T) (CCUG 45425(T)=DSM 107286(T)=BCCM/LMG 30783(T))

Harvard-Zitierstil: Sammra, O., Foster, G., Hassan, A., Alssahen, M., Lämmler, C., Glaeser, S., et al. (2020) Arcanobacterium bovis sp. nov., isolated from the milk of a cow with mastitis, International Journal of Systematic and Evolutionary Microbiology, 70(7), pp. 4105-4110. https://doi.org/10.1099/ijsem.0.004230

APA-Zitierstil: Sammra, O., Foster, G., Hassan, A., Alssahen, M., Lämmler, C., Glaeser, S., Kämpfer, P., Busse, H., Borowiak, M., Malorny, B., Ritchie, C., Prenger-Berninghoff, E., & Abdulmawjood, A. (2020). Arcanobacterium bovis sp. nov., isolated from the milk of a cow with mastitis. International Journal of Systematic and Evolutionary Microbiology. 70(7), 4105-4110. https://doi.org/10.1099/ijsem.0.004230