Journal article

Publication year: 2020

Pages: 5824-5831

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 70

Issue number: 11

ISSN: 1466-5026

eISSN: 1466-5034

Open access status: Bronze

DOI Link: https://doi.org/10.1099/ijsem.0.004482

Publisher: Microbiology Society

Abstract: 
A Gram stainingnegative non endosporeforming strain, PXU-55(T), was isolated from the rhizosphere of the switchgrass Panicum virgatum and studied in detail to determine its taxonomic position. The results of 16S rRNA gene sequence analysis indicated that the isolate represented a member of the genus Flavobacterium. The isolate shared highest 16S rRNA gene sequence similarities with the type strains of Flavobacterium chungangense (98.78 %) and Flavobacterium chilense (98.64 %). The average nucleotide identity (ANI) and in silico DNA-DNA hybridization (isDDH) values between the PXU-55(T) genome assembly and the ones of the most closely related type strains of species of the genus Flavobacterium were 87.3 and 31.9% (Flavobacterium defluvii), and 86.1 and 29.9% (Flavobacterium johnsoniae). Menaquinone MK-6 was the major respiratory quinone. As major polar lipids, phosphatidylethanolamine, an ornithine lipid and the unidentified polar lipids L2, L3 and L4 lacking a functional group were found. Moderate to minor amounts of another ornithine lipid, the unidentified lipid L1 and a glycolipid were present, as well. The major polyamine is symhomospermidine. The fatty acid profiles contained major amounts of iso-C-15:0, iso-C-15:0 3-OH, iso-C-17:0 3 OH, C-15:0, summed feature 3 (C-16:1 omega 7c and/or iso-C-15:0 2 OH) and various hydroxylated fatty acids in smaller amounts, among them iso C-16:0 3 OH, C-16:0 3 OH and C-15:0 3 OH, which supported the classification of the isolate as a member of the genus Flavobacterium. Physiological and biochemical characterisation and ANI calculations with the type strains of the most closely related species allowed a clear phenotypic and genotypic differentiation of the strain. For this reason, we propose that strain PXU-55(T) (=CIP 111646(T)=CCM 8914(T)) represents a novel species with the name Flavobacterium panici sp. nov.

Harvard Citation style: Kämpfer, P., Glaeser, S., McInroy, J., Xu, J., Busse, H., Clermont, D., et al. (2020) Flavobacterium panici sp. nov. isolated from the rhizosphere of the switchgrass Panicum virgatum, International Journal of Systematic and Evolutionary Microbiology, 70(11), pp. 5824-5831. https://doi.org/10.1099/ijsem.0.004482

APA Citation style: Kämpfer, P., Glaeser, S., McInroy, J., Xu, J., Busse, H., Clermont, D., & Criscuolo, A. (2020). Flavobacterium panici sp. nov. isolated from the rhizosphere of the switchgrass Panicum virgatum. International Journal of Systematic and Evolutionary Microbiology. 70(11), 5824-5831. https://doi.org/10.1099/ijsem.0.004482