Journal article

Publication year: 2021

Journal: International Journal of Systematic and Evolutionary Microbiology

Volume number: 71

Issue number: 10

ISSN: 1466-5026

eISSN: 1466-5034

DOI Link: https://doi.org/10.1099/ijsem.0.005043

Publisher: Microbiology Society

Abstract: 
A bacterial strain designated 32A(T) was isolated from the skin of an Anderson's salamander (Ambystoma andersoni) and subjected to a comprehensive taxonomic study. The strain was Gram-stain-negative, rod-shaped, non-motile, oxidase- and ureasenegative, and catalase-positive. 16S rRNA gene sequence comparisons placed the strain in the genus Luteolibacter with highest sequence similarities to Luteolibacter pohnpeiensis A4(T)-83(T) (95.2%), Luteolibacter gellanilyticus CB-286403(T) (95.1%) and Luteolibacter cuticulihirudinis E100T (94.9%). Genomic sequence analysis revealed a size of 5.3 Mbp, a G+C-content of 62.2 mol% and highest ANI values with Luteolibacter luteus (71.2%), Luteolibacter yonseiensis (71.4%) and L. pohnpeiensis (69.5%). In the polyamine pattern, 1,3-diaminopropane and spermidine were predominant. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The quinone system was composed of the major menaquinones MK-9 and MK-10. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, the unidentified aminolipid AL2, the unidentified phospholipid PL2 and the unidentified aminophospholipid APL1. The fatty acid profile contained major amounts of iso-C-14:0, iso-C-16:0, C-16: 0 and C16:1 omega 9c. In addition, C-14:0, C-15:0, anteiso-C-15:0, summed feature 2 (C14:0 3OH and/or iso-C-16: 0 I), and the hydroxylated fatty acids iso-C-14: 0 3OH, iso-C-16: 0 3OH and C-16: 0 3 -OH were detected. Physiologically, strain 32A(T) is distinguishable from its next relatives. Based on phylogenetic, genomic, physiological and chemotaxonomic data, strain 32A(T) represents a novel species of the genus Luteolibacter for which we propose the name Luteolibacter ambystomatis sp. nov. The type strain is 32A(T) (=CCM 9141(T)=LMG 32214(T)).

Harvard Citation style: Busse, H., Kämpfer, P., Szostak, M. and Spergser, J. (2021) Luteolibacter ambystomatis sp. nov., isolated from the skin of an Anderson’s salamander (Ambystoma andersoni), International Journal of Systematic and Evolutionary Microbiology, 71(10), Article 005043. https://doi.org/10.1099/ijsem.0.005043

APA Citation style: Busse, H., Kämpfer, P., Szostak, M., & Spergser, J. (2021). Luteolibacter ambystomatis sp. nov., isolated from the skin of an Anderson’s salamander (Ambystoma andersoni). International Journal of Systematic and Evolutionary Microbiology. 71(10), Article 005043. https://doi.org/10.1099/ijsem.0.005043