Journalartikel

Jahr der Veröffentlichung: 2022

Zeitschrift: International Journal of Systematic and Evolutionary Microbiology

Bandnummer: 72

Heftnummer: 8

ISSN: 1466-5026

eISSN: 1466-5034

DOI Link: https://doi.org/10.1099/ijsem.0.005497

Verlag: Microbiology Society

Abstract: 
A Gram-negative bacterial strain, G163CM(T), was isolated from the gut of the Asian emerald cockroach Corydidarum magnifica. The 16S rRNA gene sequence (1416 bp) of strain G163CM(T) showed 99.22% similarity to Pseudocitrobacter faecalis CCM 8479(T) and Pseudocitrobacter vendiensis CPO20170097(T).The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values of strain G163CM(T) were 92.4, 48.8 and 95.7% to P. faecalis CCM 8479(T), and 93.3, 52.4 and 95.7% to P. vendiensis CPO20170097(T). This strongly supports the designation of G163CM(T) as representing a new species in the genus Pseudocitrobacter. Phylogenetic trees based on the alignment of 16S rRNA, multilocus sequence analysis of six single -copy genes (fusA, pyrG, leuS, rpoB, recN and mnmE) and 107 core protein sequences consistently showed G163CM(T) to be a member of the genus Pseudocitrobacter, closely related to P. vendiensis CPO20170097(T.) In contrast to P. faecalis CCM 8479(T) and P. vendiensis CPO20170097(T), the genome of G163CM(T) did not encode for proteins conferring resistance to antibiotics. However, all three genomes encoded a similar number of virulence factors and specialized metabolite biosynthetic proteins. The major fatty acids of strain G163CM(T) were C-16:0 (31.5 %), C-18:1 omega 7c (22.6 %), C-17:0 cyclo (15.3 %) and C-14:0 (6.5 %). Based on the polyphasic results, we conclude that strain G163CM(T) represents a novel species of the genus Pseudocitrobacter and we propose the name Pseudocitrobacter corydidari sp. nov. with the type strain G163CM(T) (=DSM 112648(T)=CCM 9160(T)).

Harvard-Zitierstil: Guzman, J., Poehlein, A., Glaeser, S., Schwengers, O., Blom, J., Hollensteiner, J., et al. (2022) Pseudocitrobacter corydidari sp. nov., isolated from the Asian emerald cockroach Corydidarum magnifica, International Journal of Systematic and Evolutionary Microbiology, 72(8), Article 005497. https://doi.org/10.1099/ijsem.0.005497

APA-Zitierstil: Guzman, J., Poehlein, A., Glaeser, S., Schwengers, O., Blom, J., Hollensteiner, J., Kämpfer, P., & Vilcinskas, A. (2022). Pseudocitrobacter corydidari sp. nov., isolated from the Asian emerald cockroach Corydidarum magnifica. International Journal of Systematic and Evolutionary Microbiology. 72(8), Article 005497. https://doi.org/10.1099/ijsem.0.005497