Journalartikel

Jahr der Veröffentlichung: 2022

Zeitschrift: International Journal of Systematic and Evolutionary Microbiology

Bandnummer: 72

Heftnummer: 11

ISSN: 1466-5026

DOI Link: https://doi.org/10.1099/ijsem.0.005636

Verlag: Microbiology Society

Abstract: 
A Gram-stain-positive, facultative anaerobic endospore-forming bacterium, which originated from roots/rhizosphere of maize (Zea mays), was investigated for its taxonomic position. On the basis of 16S rRNA gene sequence similarities, strain JJ-3^T was grouped together with Neobacillus species showing the highest similarities to Neobacillus bataviensis (98.8 %) and the three species Neobacillus dendrensis, Neobacillus soli and Neobacillus cucumis (all 98.6 %). The 16S rRNA gene sequence similarities to the sequences of the type strains of other Neobacillus species were lower than 98.5 %. The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-3^T genome assembly and those of the other Neobacillus type strains were <83, <85 and <27 %, respectively. Chemotaxonomic features supported the grouping of the strain to the genus Neobacillus, e.g. the major fatty acids were C_15 : 0 anteiso and C_15 : 0 iso, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and the major quinone was menaquinone MK-7. Physiological and biochemical test results were slightly different from those of the most closely related species. For this reason, JJ-3^T represents a novel species of the genus Neobacillus, for which we propose the name Neobacillus rhizosphaerae sp. nov., with JJ-3^T (= CIP 111895^T=LMG 32087^T=DSM 111784^T=CCM 9084^T) as the type strain. We also propose to reclassify Bacillus dielmonensis as Neobacillus dielmonensis comb. nov. based mainly on the results of phylogenomic and conserved signature indel analyses.

Harvard-Zitierstil: Kämpfer, P., Glaeser, S., McInroy, J., Clermont, D., Lipski, A. and Criscuolo, A. (2022) Neobacillus rhizosphaerae sp. nov., isolated from the rhizosphere, and reclassification of Bacillus dielmonensis as Neobacillus dielmonensis comb. nov., International Journal of Systematic and Evolutionary Microbiology, 72(11), Article 005636. https://doi.org/10.1099/ijsem.0.005636

APA-Zitierstil: Kämpfer, P., Glaeser, S., McInroy, J., Clermont, D., Lipski, A., & Criscuolo, A. (2022). Neobacillus rhizosphaerae sp. nov., isolated from the rhizosphere, and reclassification of Bacillus dielmonensis as Neobacillus dielmonensis comb. nov.. International Journal of Systematic and Evolutionary Microbiology. 72(11), Article 005636. https://doi.org/10.1099/ijsem.0.005636