Journal article
Authors list: Riedel, C; Lamp, B; Hagen, B; Indik, S; Rümenapf, T
Publication year: 2017
Journal: Scientific Reports
Volume number: 7
ISSN: 2045-2322
DOI Link: https://doi.org/10.1038/srep44459
Publisher: Nature Research
A multitude of viral factors - either inhibiting the induction of the IFN-system or its effectors - have been described to date. However, little is known about the role of structural components of the incoming virus particle in protecting against IFN-induced antiviral factors during or immediately after entry. In this study, we take advantage of the previously reported property of Classical swine fever virus (family Flaviviridae, genus Pestivirus) to tolerate a deletion of the core protein if a compensatory mutation is present in the NS3-helicase-domain (Vp447(triangle c)). In contrast to the parental virus (Vp447), which causes a hemorrhagic-fever-like disease in pigs, Vp447(triangle c) is avirulent in vivo. In comparison to Vp447, growth of Vp447(triangle c) in primary porcine cells and IFN-treated porcine cell lines was reduced > 20-fold. Also, primary porcine endothelial cells and IFN-pretreated porcine cell lines were 8-24 times less susceptible to Vp447(triangle c). This reduction of susceptibility could be partially reversed by loading Vp447(triangle c) particles with different levels of core protein. In contrast, expression of core protein in the recipient cell did not have any beneficial effect. Therefore, a protective effect of core protein in the incoming virus particle against the products of IFN-stimulated genes could be demonstrated.
Abstract:
Citation Styles
Harvard Citation style: Riedel, C., Lamp, B., Hagen, B., Indik, S. and Rümenapf, T. (2017) The core protein of a pestivirus protects the incoming virus against IFN-induced effectors, Scientific Reports, 7, Article 44459. https://doi.org/10.1038/srep44459
APA Citation style: Riedel, C., Lamp, B., Hagen, B., Indik, S., & Rümenapf, T. (2017). The core protein of a pestivirus protects the incoming virus against IFN-induced effectors. Scientific Reports. 7, Article 44459. https://doi.org/10.1038/srep44459
