Journal article

Publication year: 2012

Pages: 2742-2748

Journal: Vaccine

Volume number: 30

Issue number: 17

ISSN: 0264-410X

DOI Link: https://doi.org/10.1016/j.vaccine.2012.02.029

Publisher: Elsevier

Abstract: 
Live attenuated C-strain classical swine fever viruses (CSFV) provide a rapid onset of protection, but the lack of a serological test that can differentiate vaccinated from infected animals limits their application in CSF outbreaks. Since immunity may precede antibody responses, we examined the kinetics and specificity of peripheral blood T cell responses from pigs vaccinated with a C-strain vaccine and challenged after five days with a genotypically divergent CSFV isolate. Vaccinated animals displayed virus-specific IFN-gamma responses from day 3 post-challenge, whereas, unvaccinated challenge control animals failed to mount a detectable response. Both CD4(+) and cytotoxic CD8(+) T cells were identified as the cellular source of IFN-gamma. IFN-gamma responses showed extensive cross-reactivity when T cells were stimulated with CSFV isolates spanning the major genotypes. To determine the specificity of these responses, T cells were stimulated with recombinant CSFV proteins and a proteome-wide peptide library from a related virus, BVDV. Major cross-reactive peptides were mapped on the E2 and NS3 proteins. Finally, IFN-gamma was shown to exert potent antiviral effects on CSFV in vitro. These data support the involvement of broadly cross-reactive T cell IFN-gamma responses in the rapid protection conferred by the C-strain vaccine and this information should aid the development of the next generation of CSFV vaccines. Crown Copyright (c) 2012 Published by Elsevier Ltd. All rights reserved.

Harvard Citation style: Graham, S., Haines, F., Johns, H., Sosan, O., Rocca, S., Lamp, B., et al. (2012) Characterisation of vaccine-induced, broadly cross-reactive IFN-γ secreting T cell responses that correlate with rapid protection against classical swine fever virus, Vaccine, 30(17), pp. 2742-2748. https://doi.org/10.1016/j.vaccine.2012.02.029

APA Citation style: Graham, S., Haines, F., Johns, H., Sosan, O., Rocca, S., Lamp, B., Rümenapf, T., Everett, H., & Crooke, H. (2012). Characterisation of vaccine-induced, broadly cross-reactive IFN-γ secreting T cell responses that correlate with rapid protection against classical swine fever virus. Vaccine. 30(17), 2742-2748. https://doi.org/10.1016/j.vaccine.2012.02.029