Journal article

Publication year: 2012

Pages: 497-502

Journal: Biochemical Journal

Volume number: 444

Issue number: 3

ISSN: 0264-6021

eISSN: 1470-8728

DOI Link: https://doi.org/10.1042/BJ20112166

Publisher: Portland Press

Abstract: 
Candida albicans RCH1 (regulator of Ca2+ homoeostasis 1) encodes a protein of ten TM (transmembrane) domains, homologous with human SLC10A7 (solute carrier family 10 member 7), and Rchlp localizes in the plasma membrane. Deletion of RCH1 confers hypersensitivity to high concentrations of extracellular Ca2+ and tolerance to azoles and L+, which phenocopies the deletion of CaPMC1 (C. albicans PMC1) encoding the vacuolar Ca2+ pump. Additive to CaPMC1 mutation, lack of RCH1 alone shows an increase in Ca2+ sensitivity, Ca2+ uptake and cytosolic Ca2+ level. The Ca2+ hypersensitivity is abolished by cyclosporin A and magnesium.In addition, deletion of RCH1 elevates the expression of CaUTR2 (C. albi cans UTR2), a downstream target of the Ca2+ /calcineurin signalling. Mutational and functional analysis indicates that the Rch1p TM8 domain, but not the TM9 and TM10 domains, are required for its protein stability, cellular functions and subcellular localization. Therefore Rch1p is a novel regulator of cytosolic Ca2+ homoeostasis, which expands the functional spectrum of the vertebrate SLC10 family.

Harvard Citation style: Jiang, L., Alber, J., Wang, J., Du, W., Yang, X., Li, X., et al. (2012) The Candida albicans plasma membrane protein Rch1p, a member of the vertebrate SLC10 carrier family, is a novel regulator of cytosolic Ca2+ homoeostasis, Biochemical Journal, 444(3), pp. 497-502. https://doi.org/10.1042/BJ20112166

APA Citation style: Jiang, L., Alber, J., Wang, J., Du, W., Yang, X., Li, X., Sanglard, D., & Geyer, J. (2012). The Candida albicans plasma membrane protein Rch1p, a member of the vertebrate SLC10 carrier family, is a novel regulator of cytosolic Ca2+ homoeostasis. Biochemical Journal. 444(3), 497-502. https://doi.org/10.1042/BJ20112166