Journalartikel

Jahr der Veröffentlichung: 2021

Seiten: 187-195

Zeitschrift: Nature Microbiology

Bandnummer: 6

Heftnummer: 2

ISSN: 2058-5276

Open Access Status: Green

DOI Link: https://doi.org/10.1038/s41564-020-00815-6

Verlag: Nature Research

Abstract: 

A DNA-based vaccine elicits humoral and cellular immunity and provides protection against Crimean-Congo haemorrhagic fever virus-mediated disease in a non-human primate model.
There is currently no specific prophylaxis or vaccine against Crimean-Congo haemorrhagic fever virus (CCHFV). Crimean-Congo haemorrhagic fever (CCHF) is a severe febrile illness transmitted by Hyalomma ticks in endemic areas, handling of infected livestock or care of infected patients. We report here the successful protection against CCHFV-mediated disease in a non-human primate disease model. Cynomolgus macaques were vaccinated with a DNA-based vaccine using in vivo electroporation-assisted delivery. The vaccine contained two plasmids encoding the glycoprotein precursor (GPC) and the nucleoprotein (NP) of CCHFV. Animals received three vaccinations and we recorded potent antibody and T cell responses after vaccination. While all sham-vaccinated animals developed viraemia, high tissue viral loads and CCHF-induced disease, the NP + GPC vaccinated animals were significantly protected. In conclusion, this is evidence of a vaccine that can protect against CCHFV-induced disease in a non-human primate model. This supports clinical development of the vaccine to protect groups at risk for contracting the infection.

Harvard-Zitierstil: Hawman, D., Ahlén, G., Appelberg, K., Meade-White, K., Hanley, P., Scott, D., et al. (2021) A DNA-based vaccine protects against Crimean-Congo haemorrhagic fever virus disease in a Cynomolgus macaque model, Nature Microbiology, 6(2), pp. 187-195. https://doi.org/10.1038/s41564-020-00815-6

APA-Zitierstil: Hawman, D., Ahlén, G., Appelberg, K., Meade-White, K., Hanley, P., Scott, D., Monteil, V., Devignot, S., Okumura, A., Weber, F., Feldmann, H., Sällberg, M., & Mirazimi, A. (2021). A DNA-based vaccine protects against Crimean-Congo haemorrhagic fever virus disease in a Cynomolgus macaque model. Nature Microbiology. 6(2), 187-195. https://doi.org/10.1038/s41564-020-00815-6