Journalartikel

Jahr der Veröffentlichung: 2020

Seiten: 10397-10412

Zeitschrift: Nucleic Acids Research

Bandnummer: 48

Heftnummer: 18

ISSN: 0305-1048

eISSN: 1362-4962

Open Access Status: Gold

DOI Link: https://doi.org/10.1093/nar/gkaa739

Verlag: Oxford University Press

Abstract: 

The RNA helicase RIG-I plays a key role in sensing pathogen-derived RNA. Double-stranded RNA structures bearing 5′-tri- or diphosphates are commonly referred to as activating RIG-I ligands. However, endogenous RNA fragments generated during viral infection via RNase L also activate RIG-I. Of note, RNase-digested RNA fragments bear a 5′-hydroxyl group and a 2′,3′-cyclic phosphate. How endogenous RNA fragments activate RIG-I despite the lack of 5′-phosphorylation has not been elucidated. Here we describe an endogenous RIG-I ligand (eRL) that is derived from the internal transcribed spacer 2 region (ITS2) of the 45S ribosomal RNA after partial RNase A digestion in vitro, RNase A protein transfection or RNase L activation. The immunostimulatory property of the eRL is dependent on 2′,3′-cyclic phosphate and its sequence is characterized by a G-quadruplex containing sequence motif mediating guanosine-5′-triphosphate (GTP) binding. In summary, RNase generated self-RNA fragments with 2′,3′-cyclic phosphate function as nucleotide-5′-triphosphate binding aptamers activating RIG-I.

Harvard-Zitierstil: Jung, S., von Thulen, T., Yang, I., Laukemper, V., Rupf, B., Janga, H., et al. (2020) A ribosomal RNA fragment with 2′,3′-cyclic phosphate and GTP-binding activity acts as RIG-I ligand, Nucleic Acids Research, 48(18), pp. 10397-10412. https://doi.org/10.1093/nar/gkaa739

APA-Zitierstil: Jung, S., von Thulen, T., Yang, I., Laukemper, V., Rupf, B., Janga, H., Panagiotidis, G., Schoen, A., Nicolai, M., Schulte, L., Obermann, H., Weber, F., Kaufmann, A., & Bauer, S. (2020). A ribosomal RNA fragment with 2′,3′-cyclic phosphate and GTP-binding activity acts as RIG-I ligand. Nucleic Acids Research. 48(18), 10397-10412. https://doi.org/10.1093/nar/gkaa739