Journal article
Authors list: Klingelhöfer, Ines; Ngoc, Long Pham; van der Burg, Bart; Morlock, Gertrud E.
Publication year: 2021
Journal: Analytica Chimica Acta
Volume number: 1183
ISSN: 0003-2670
eISSN: 1873-4324
DOI Link: https://doi.org/10.1016/j.aca.2021.338956
Publisher: Elsevier
For the first time, a human cancer cell line was shown to grow and be functionally active on the particulate porous adsorbent surface of separated sample mixtures. This allowed the novel combination of chromatographic separations with human cells as biological detector. As exemplary screening for cancer treatment drugs, cytotoxic substances were directly discovered in Saussurea costus and ginseng samples using the Cytotox CALUX (R) osteosarcoma cells (with luciferase expressing reporter gene) as detector. In addition, rosiglitazone and pioglitazone were detected as luminescent zones upon binding to the PPAR gamma receptor expressed in the respective CALUX cell line that was grown on the surface of the adsorbent. This demonstrates the ability to address receptor-mediated signaling with this method, and opens the perspective to use our novel bioimaging method to identify bioactive molecules targeting a wide range of pathways with toxicological, pharmaceutical and nutraceutical relevance. The new bioimaging directly pointed to individual effective compounds in multi-component mixtures. Furthermore, discovered effective compounds were directly characterized by online elution to high-resolution mass spectrometry and fragmentation.
Abstract:
Citation Styles
Harvard Citation style: Klingelhöfer, I., Ngoc, L., van der Burg, B. and Morlock, G. (2021) A bioimaging system combining human cultured reporter cells and planar chromatography to identify novel bioactive molecules, Analytica Chimica Acta, 1183, Article 338956. https://doi.org/10.1016/j.aca.2021.338956
APA Citation style: Klingelhöfer, I., Ngoc, L., van der Burg, B., & Morlock, G. (2021). A bioimaging system combining human cultured reporter cells and planar chromatography to identify novel bioactive molecules. Analytica Chimica Acta. 1183, Article 338956. https://doi.org/10.1016/j.aca.2021.338956
