Multiplex planar bioassay with reduced diffusion on normal phase, identifying androgens, verified antiandrogens and synergists in botanicals via 12D hyphenation - Research portal of Justus Liebig University Giessen:

Journal article

Multiplex planar bioassay with reduced diffusion on normal phase, identifying androgens, verified antiandrogens and synergists in botanicals via 12D hyphenation

Authors list: Schreiner, T.; Ronzheimer, A.; Friz, M.; Morlock, G. E.

Publication year: 2022

Journal: Food Chemistry

Volume number: 395

ISSN: 0308-8146

eISSN: 1873-7072

DOI Link: https://doi.org/10.1016/j.foodchem.2022.133610

Publisher: Elsevier

Abstract:

Hormonal active compounds affecting health by altering the hormonal system are present in food. The planar yeast antagonist androgen screen (pYAAS) bioassay is a powerful tool to detect individual hormonal active compounds in complex samples separated by high-performance thin-layer chromatography (HPTLC). Previous methods lacked either detection sensitivity or zone sharpness. To overcome diffusion caused by long bioassay incubation on the normal-phase (NP) plate, zone fixation ((fix)) was achieved with a new polyisobutyl methacrylate coating, leading to enhanced zone sharpness. The exclusion of false-positive antagonists was integrated in the workflow, which allowed the verification (V) of true antagonists, apart from the detection of synergists. With the new multiplex bioassay providing information on 4 activities, 68 different botanicals were screened and hormonal active zones were identified by elution from the bioautogram to orthogonal reversed-phase high performance liquid chromatography with diode array detection and high-resolution mass spectrometry including fragmentation, resulting in the 12D hyphenation NP-HPTLCfix-UV/Vis/FLD-pYAVAS-FLD-heart cut-RP-HPLC-DAD-HRMS/MS.

Authors/Editors

- Uni.-Prof. Dr. Gertrud Elisabeth Morlock

Citation Styles

Harvard Citation style: Schreiner, T., Ronzheimer, A., Friz, M. and Morlock, G. (2022) Multiplex planar bioassay with reduced diffusion on normal phase, identifying androgens, verified antiandrogens and synergists in botanicals via 12D hyphenation, Food Chemistry, 395, Article 133610. https://doi.org/10.1016/j.foodchem.2022.133610

APA Citation style: Schreiner, T., Ronzheimer, A., Friz, M., & Morlock, G. (2022). Multiplex planar bioassay with reduced diffusion on normal phase, identifying androgens, verified antiandrogens and synergists in botanicals via 12D hyphenation. Food Chemistry. 395, Article 133610. https://doi.org/10.1016/j.foodchem.2022.133610

SDG Areas

6 Clean water and sanitation