Journal article

Publication year: 2016

Pages: 389-400

Journal: Berliner und Münchener Tierärztliche Wochenschrift

Volume number: 129

Issue number: 9-10

ISSN: 0005-9366

eISSN: 1439-0299

DOI Link: https://doi.org/10.2376/0005-9366-15077

Publisher: Schlütersche Verlagsgesellschaft

Abstract: 

Input and output samples of ten German biogas plants (BGPs) were investigated for the presence of extended-spectrum beta-lactamase (ESBL) carrying Escherichia coli (ESBL E. coli), vancomycin resistant enterococci (VRE), and methicillin resistant staphylococci (MRS). After selective pre-enrichments from input and output material, ESBL E. coli and VRE were isolated from both, MRS only from input samples. However, cultivation independent analysis showed also the presence of mecA genes in the output samples. Isolated ESBL E. coli (n = 34) were assigned to E. coli phylogroups A and B1 and six MLST sequence types, among those two known STs, ST117 and ST641. Among eleven isolated VRE, two vanA gene carrying isolates from an output sample were most closely related to the clinically relevant species E. faecium (100%) and identified as E. faecium MLST sequence type ST80 of the clonal complex CC17. Other vanA or vanB gene carrying VRE were most closely related to the type strains of E. viikkiensis/E. pseudoavium/E. devriesei, E. mundtii or E. casseliflavum (99.9% 16S rRNA gene sequence identity). The seven mecA carrying MRS isolated from two input samples showed highest similarities to type strains of S. haemolyticus, S. cohnii, and S. lentus (99.7 to 100% 16S rRNA gene sequence similarity). Methicillin-resistant S. aureus (MRSA) were not detected. In summary, the study showed, that mesophilic BGPs fed by manure from livestock husbandry can release ESBL E. coli and VRE and/or ESBL, vancomycin and methicillin resistance genes into the environment.

Harvard Citation style: Glaeser, S., Schauss, T., Brunner, J., Sowinsky, O., Breves, G. and Kämpfer, P. (2016) Pilot study for the detection of extended-spectrum beta-lactamase (ESBL) carrying Escherichia coli, vancomycin resistant enterococci (VRE) and methicillin resistant staphylococci (MRS) in input and output samples of German biogas plants, Berliner und Münchener Tierärztliche Wochenschrift, 129(9-10), pp. 389-400. https://doi.org/10.2376/0005-9366-15077

APA Citation style: Glaeser, S., Schauss, T., Brunner, J., Sowinsky, O., Breves, G., & Kämpfer, P. (2016). Pilot study for the detection of extended-spectrum beta-lactamase (ESBL) carrying Escherichia coli, vancomycin resistant enterococci (VRE) and methicillin resistant staphylococci (MRS) in input and output samples of German biogas plants. Berliner und Münchener Tierärztliche Wochenschrift. 129(9-10), 389-400. https://doi.org/10.2376/0005-9366-15077